摘要
目的探讨二氢杨梅素对2型糖尿病小鼠非酒精性脂肪肝的保护作用及对肠道菌群的影响。方法雄性C57BL/6J小鼠采用高脂饲料喂饲联合腹腔注射链脲佐菌素构建2型糖尿病小鼠模型,建模成功后小鼠随机分为高血糖对照组(HG组,n=10)、低剂量DHM(HG-L组,n=10)及高剂量DHM(HG-H组,n=10),分别喂饲高脂饲料及添加低剂量DHM(500mg/kg)或高剂量DHM(1000mg/kg)的高脂饲料。正常血糖对照组(NG组,n=10)小鼠喂饲普通对照饲料。干预8w后检测小鼠血脂水平和肝脏脂质含量,并用16S rRNA高通量测序分析粪便中的肠道菌群,用气相色谱法检测粪便中短链脂肪酸的含量。结果与NG对照组相比,HG组小鼠肝脏脂质沉积增加,血浆和肝脏TG和TC水平均显著升高(P<0.05)。二氢杨梅素干预后,小鼠血浆和肝脏TG和TC水平均有所降低,其中HG-H组与HG组相比,差异具有统计学意义(P<0.05)。HG-H组小鼠血浆AST和ALT水平较HG组显著降低(P<0.05)。对肠道菌群分析发现,二氢杨梅素干预对小鼠肠道菌群组成有影响,在科水平上,HG-H组小鼠粪便中乳杆菌科(Lactobacillaceae)和螺杆菌科(Helicobacteraceae)的相对丰度降低,而毛螺菌科(Lachnospiraceae)的相对丰度升高,与HG组相比,差异具有统计学意义(P<0.05)。在属水平上,二氢杨梅素干预组HG-L和HG-H组小鼠粪便中乳酸菌属(Lactobacillus)和布劳特氏菌属(Blautia)的相对丰度明显升高,同时小鼠粪便中乙酸和丙酸的含量显著增加。结论二氢杨梅素可缓解糖尿病小鼠非酒精性脂肪肝,其可能的作用机制是通过调节小鼠肠道菌群的结构组成,并增加其代谢产物短链脂肪酸的生成。
Objective To investigate the effects of dihydromyricetin(DHM)on non-alcoholic fatty liver in type 2diabetic(T2DM)mice,and further explore its effects on gut microbiota.Methods T2DM mice with non-alcoholic fatty liver disease was induced by high fat diet(HFD)and streptozocin(STZ).After the model was established successfully,the diabetic mice were randomly divided into three groups:diabetic group(HG,n=10)were fed HFD,HG-L group(n=10)were fed HFD supplemented with 500mg/kg DHM,HG-H group(n=10)were fed HFD supplemented with 1000 mg/kg DHM.The control group(NG,n=10)received normal rodent chow.After 8 weeks,plasma levels of total cholesterol(TC),triglyceride(TG),high-density lipoprotein cholesterol(HDL-C)and low-density lipoprotein cholesterol(LDL-C),liver lipid contents as well as and the activities of aspartate amino transferase(AST)and alanine amino transferase(ALT)were determined.Furthermore,16S high-throughput sequencing technology was used to analyze the changes in gut microbiota and gas chromatography-mass spectrometry used to detect the contents of short chain fatty acids(SCFAs)in the feces.Results Compared with the control group,the liver tissue of mice from the HFD group presented a large amount of lipid deposition,and the levels of TG and TC in plasma and liver increased significantly(P<0.05).Dihydromyricetin significantly reduced plasma levels of TC,TG,ALT and AST in diabetic mice,and the difference between HG-H group and HG group was statistically significant(P<0.05).The levels of plasma AST and ALT in the HG-H group were significantly lower than those in the HG group(P<0.05).Moreover,the contents of TC and TG in the liver were also significantly reduced.The analysis of intestinal flora demonstrated that dihydromyricetin treatment significantly changed the composition of gut microbiota in mice.At the family level,the relative abundance of Lactobacillaceae and Helicobacteraceae in the feces of the HG-H group was significantly decreased,while the relative abundance of Lachnospiraceae was significantly increa
作者
宋丰林
徐艺璇
杨燕
毛钰蘅
SONG Feng-lin;XU Yi-xuan;YANG Yan;MAO Yu-hen(College of Food Science,Guangdong Pharmaceutical University,Zhongshan 528458,China;GDPU-HKU Zhongshan Biomedical Innovation Platform,Zhongshan 528458,China;School of Public Health,Shenzhen,Sun Yat-sen University,Shenzhen 518106,China;Guangdong Provincial Key Laboratory of Food,Nutrition and Health,Guangzhou 510080,China;Guangdong Engineering Technology Research Center of Nutrition Translation,Guangzhou 510080,China)
出处
《营养学报》
CAS
CSCD
北大核心
2022年第6期598-606,共9页
Acta Nutrimenta Sinica
基金
中山市社会公益与基础研究项目重点项目(No.2020B2012)
国家自然科学基金青年科学基金项目(No.81502808)
国家自然科学基金重点项目(No.82030098)
国家自然科学基金面上项目(No.81872617)
广东省营养膳食与健康重点实验室开放基金项目(No.2019-GLFNH-04)。
关键词
二氢杨梅素
糖尿病
非酒精性脂肪肝
肠道菌群
短链脂肪酸
dihydromyricetin
diabetes
non-alcoholic fatty liver disease
gut microbiota
short-chain fatty acids
