摘要
有机磷农药残留危害环境和人类健康。本文采用电沉积法将乙酰胆碱酯酶(AChE)与胆碱氧化酶(COD)固定化,共修饰于裸金电极表面,构建新型固定化酶生物传感器。通过电子显微镜对固定化酶形貌进行表征,结果表明:当固定化酶生物传感器上,乙酰胆碱酯酶与乙酰胆碱氧化酶的共修饰层数为2层,修饰圈数为35圈,在氯化乙酰胆碱浓度为2 mmol/L,pH 7.8的检测体系中,该生物传感器能够识别有机磷农药信号,且检测性能尚佳。传感器在有机磷农药质量浓度为10^(-9)~10^(-7)mg/L时电流响应良好,工作曲线方程为y=0.0468x+1.2124(R^(2)=0.9985),最低检出限1.15×10^(-11)mg/L(S/N=3)。本研究为现场快速检测有机磷农药提供了新的研究方法。
In this paper,it reported an acetylcholinesterase(ACh E)and choline oxidase(COD)were immobilized and co-modified on the bare gold electrode surface by electrodeposition to construct a novel immobilized enzyme biosensor.The morphology of immobilized enzyme was characterized by electron microscope.The results showed that the comodification layers of immobilized acetylcholinesterase and immobilized acetylcholinesterase in immobilized enzyme biosensor were 2 layers and the number of modification cycles was 35.When the concentration of acetylcholine chloride substrate is 2 mmol/L and p H value is 7.8,the biosensor can recognize the organic phosphorus pesticide signal and has a good detection performance,with a good sensor current response at the organic phosphorus pesticide concentration of 10^(-9)to 10^(-7)mg/L.The working curve equation is y=0.0468x+1.2124(R^(2)=0.9985),and the lowest detection limit is 1.15×10^(-11)mg/L(S/N=3).This research method will provide a new research direction for the rapid detection of organophosphorus pesticides in the field.
作者
王艳
赵宁
李虹佳
王悦
辛嘉英
张娜
Wang Yan;Zhao Ning;Li Hongjia;Wang Yue;Xin Jiaying;Zhang Na(College of Food Engineering,Harbin University of Commerce,Harbin 150076)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2023年第1期356-363,共8页
Journal of Chinese Institute Of Food Science and Technology
基金
黑龙江省自然科学基金项目(LH2021C051)
黑龙江省“百千万”工程科技重大专项支撑行动计划项目子课题(2021ZX12B07-2)
中央支持地方高校改革发展资金人才培养支持计划项目(高水平人才)(304017)。
关键词
有机磷农药
双酶共固定化
反定量检测传感器
有机磷快检
organophosphorus pesticide
double enzyme co-immobilization
reverse quantitative detection sensor
organophosphorus quick inspection
