摘要
本文基于响应曲面法以优化人工栽培川贝母的热风干燥工艺,采用单因素实验方法考察水分转换点,在此基础上利用响应面法设计优化栽培川贝母二段式变温热风干燥加工工艺,采用HPLC建立栽培川贝母核苷类成分的含量测定方法,以药材性状得分、总生物碱、总淀粉、总核苷含量归一化值(OD值)为指标建立模型对药材质量评价。通过对比恒温干燥与分段式热风烘干药材的外在性状与内在质量,对筛选得到的最佳分段式热风干燥工艺进行验证。最终得到栽培川贝母的最佳热风干燥工艺:60℃干燥至含水量45%,取出放置12 h,于50℃干燥至含水量≤15.0%。实验结果表明该工艺具有可靠性与稳定性,在此条件下,加工得到的栽培川贝母外观性状及内在质量均较优,可用于栽培川贝母药材实际生产。
In this paper,based on response surface method to optimize the hot air drying process of cultivated Fritillria cirrhosa D.Don,moisture transition point by single factor experiment,on the basis of the response surface method is used to design optimization cultivated F.cirrhosa two-stage variable warm wind drying process,the use of HPLC to establish cultivated F.cirrhosa nucleoside compounds content determination method,a model was established to evaluate the quality of medicinal materials by taking the character score of medicinal materials,the normalized(OD value)of the contents of total alkaloids,total starch and total nucleosides as indicators.By comparing the external characters and internal quality of the medicinal materials of constant temperature drying and sectional hot air drying,the optimal sectional hot air drying process was verified.Finally,the best hot air drying process of cultivated F.cirrhosa was obtained:drying at 60℃to 45%water content,taking out and placing for 12 h,drying at 50℃to less than 15.0%water content.The experimental results showed that the process was reliable and stable.Under this condition,the appearance and internal quality of cultivated F.cirrhosa were better,which could be used in the actual production of cultivated F.cirrhosa.
作者
种叶敏
李巧
杨转珍
杨永泓
蒋孟莲
蔡晓洋
李敏
黄勇
CHONG Ye-min;LI Qiao;YANG Zhuan-zhen;YANG Yong-hong;JIANG Meng-lian;CAI Xiao-yang;LI Min;HUANG Yong(State Key Laboratory of Southwestern Chinese Medicine Resources,School of Pharmacy/School of Modern Chinese Medicine Industry,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;Sichuan Lulin Fritillariae Cirrhosae Bulbu Planting Co.,Ltd.,Liangshan Yi Autonomous Prefecture 615000,China)
出处
《天然产物研究与开发》
CAS
CSCD
2023年第1期95-107,共13页
Natural Product Research and Development
基金
四川省科技厅重点研发项目(2020YFS0303)。
关键词
栽培川贝母
分段式变温
热风干燥
响应面设计
核苷
加工工艺
cultivation of F.cirrhosa
segmented temperature change
hot air drying
response surface design
nucleoside
processing technology
