摘要
千层塔中脂肪族类化学成分及其抗肿瘤活性研究。在肿瘤抑制活性引导下,采用AB-8大孔树脂、氧化铝、硅胶、Sephadex LH 20和半制备液相等方法,对千层塔甲醇提取物进行较系统分离纯化,并结合现代波谱手段对分离到的化合物进行结构鉴定。共分离鉴定了9个单体化合物,分别为pentyl-4-oxoicosa-2(Z),6(Z)-dienoate(1)、2-(2-ethoxy-1-hydroxy-2-oxoethyl)-2-hydroxy-4-methylpentanoic acid(2)、ethyl (9Z,12Z)-octadeca-9,12-dienoate(3)、(Z)-nonadec-10-enal(4)、油酸酰胺(5)、反式-9-十八碳烯酸(6)、bacillamidin A(7)、(3Z,6Z,9Z)-3,6,9-二十二碳三烯(8)、oplopandiol(9)。其中,化合物1和化合物2为新化合物,化合物3~9系首次从千层塔中分离。肿瘤抑制活性结果显示,化合物1和3对HepG2细胞具有抑制作用,其IC50分别为41.12、80.54μmol/L。
The anti-tumor activity of aliphatic in Huperzia serrata was studied.Guided by antitumor activity, the AB-8 macroporous resin, alumina, silica gel, Sephadex LH 20 and pre-HPLC were used to separate and purify the methanol extract of H.serrata.The structures were identified by modern spectroscopic methods.Nine compounds were isolated and identified, which were classified as pentyl-4-oxoicosa-2(Z),6(Z)-dienoate(1),2-(2-ethoxy-1-hydroxy-2-oxoethyl)-2-hydroxy-4-methylpentanoic acid(2),ethyl(9Z,12Z)-octadeca-9,12-dienoate(3),(Z)-nonadec-10-enal(4),oleamide(5),trans-octadec-9-enoic acid(6),bacillamidin A(7),(3Z,6Z,9Z)-3,6,9-docosatriene(8),oplopandiol(9).Compounds 1 and 2 were new compounds.Compounds 3-9 were isolated from H.serrata for the first time.The results of HepG2 cells inhibitory activity showed that compounds 1 and 3 had inhibitory effect on HepG2 with IC50of 41.12 and 80.54 μmol/L,respectively.
作者
曹朵
李佳慧
侯梦阳
王燕
李涛
CAO Duo;LI Jia-hui;HOU Meng-yang;WANG Yan;LI Tao(School of Life Sciences,Yan′an University;Key Laboratory of Jujube,Yan′an University,Yan′an 716000,China;School of Medicine,Anhui University of Science and Technology,Huainan 232000,China;School of Bioengineering,Dalian University of Technology,Dalian 116000,China;Liqun Hospital,Putuo District,Shanghai,Shanghai 200333,China)
出处
《天然产物研究与开发》
CAS
CSCD
2023年第1期40-46,共7页
Natural Product Research and Development
基金
陕西省科学技术协会青年人才托举计划(20220321)
延安大学疫情防控应急科研项目(ydfk067)
上海市普陀区卫生健康系统科技创新项目(ptkwws20 2118)。
关键词
千层塔
脂肪族类化学成分
抗肿瘤
Huperzia serrata
aliphatic compounds
anti-tumor activity
