摘要
目的 子宫内膜癌(Endometrial Cancer, EC)是女性生殖系统常见的恶性肿瘤,但其发生发展的分子机制目前尚不十分明确。MOB1B(Mps One Binder Kinase Activator 1B)是Hippo通路激酶级联反应的核心组成部分,是一个肿瘤抑制因子,而其在EC中的分子作用尚未有文献报道。尝试阐明MOB1B对EC细胞生物学行为的影响及可能的分子机制,将为临床治疗EC提供新的实验依据和理论基础。方法 利用starBase数据库分析MOB1B、CCND1(Cyclin D1)和XIAP(X-linked inhibitor of apoptosis)在EC中的mRNA表达水平,以及运用在线生物信息学软件Kaplan-Meier Plotter分析MOB1B的表达水平与EC患者生存率的关系;qRT-PCR和Western Blot检测EC细胞系Ishikawa中转染MOB1B过表达质粒后MOB1B、CCND1和XIAP的mRNA和蛋白表达水平;CCK-8、细胞集落形成、Transwell和划痕实验检测细胞的增殖、侵袭和迁移能力。结果 starBase数据库分析发现:在EC患者肿瘤组织中MOB1B的mRNA表达水平显著降低(P<0.05);利用Kaplan-Meier Plotter数据库进一步分析后发现:MOB1B低表达的EC患者生存率明显降低(P<0.05);在EC细胞中转染MOB1B过表达质粒后,MOB1B的mRNA和蛋白表达水平显著升高,而促癌因子CCND1和XIAP的mRNA和蛋白表达水平显著降低(P<0.05);与对照组相比,过表达MOB1B后EC细胞的增殖、侵袭和迁移能力被显著抑制(P<0.05)。结论 MOB1B过表达可抑制EC的细胞增殖、侵袭和迁移能力,其分子机制可能与抑制促癌因子CCND1和XIAP的表达有关。
Objective Endometrial cancer(EC) is a common malignant tumor of the female reproductive system, but the molecular mechanism of its occurrence and development is still unclear. MOB1 B(Mps One Binder Kinase Activator 1 B), a core component of Hippo pathway, is a tumor suppressor, but its molecular role in EC has not been reported. Attempts to elucidate the effects of MOB1 B on EC cells biological behavior and the possible mechanisms will provide new experimental and theoretical basis for clinical treatment of EC. Methods The mRNA expression levels of MOB1 B, Cyclin D1 and X-linked inhibitor of Apoptosis(XIAP) in EC were analyzed by starBase database. Kaplan-meier Plotter database was used to analyze the relationship between the expression level of MOB1 B and the survival rate of EC patients. The mRNA and protein expression levels of MOB1 B, CCND1 and XIAP after transfection of MOB1 B overexpressed plasmid in EC cells Ishikawa were detected by qRT-PCR and Western Blot. Cell counting kit-8(CCK-8), cell colony formation, Transwell, and wound healing assay were used to detect cell proliferation, invasion, and migration. Results starBase database analysis showed that the mRNA expression level of MOB1 B in EC patients was significantly decreased(P<0.05). Further analysis using Kaplan-Meier Plotter database showed that the survival rate of EC patients with low MOB1 B expression was significantly decreased(P<0.05). After transfection of MOB1 B overexpressed plasmid into EC cells, the mRNA and protein expression levels of MOB1 B were significantly increased, while the mRNA and protein expression levels of CCND1 and XIAP were significantly decreased(P<0.05). Compared with the control group, the proliferation, invasion and migration of EC cells were significantly inhibited after MOB1 B overexpression(P<0.05). Conclusion MOB1 B overexpression can inhibit EC cells proliferation, invasion and migration, and the molecular mechanism may be related to the inhibition of CCND1 and XIAP expression.
作者
庞槐
王竞州
杨冰琪
袁成钢
魏茜茜
刘杰
谢建新
PANG Huai;WANG Jingzhou;YANG Bingqi;YUAN Chenggang;WEI Qianqian;LIU Jie;XIE Jianxin(School of Medicine,Shihezi University,Shihezi,Xinjiang 832000,China)
出处
《石河子大学学报（自然科学版）》
CAS
北大核心
2022年第6期772-778,共7页
Journal of Shihezi University(Natural Science)
基金
国家自然科学基金项目(82160496)
新疆维吾尔自治区研究生教育创新项目(XJ2021G125)。
