摘要
目的探讨着丝粒蛋白K基因(CENPK基因)在前列腺癌中的表达及其临床意义。方法通过下载癌症基因组图谱(TCGA)和基因表达谱数据交互分析(GEPIA)数据库相关数据,获得498例前列腺癌患者的mRNA表达水平及临床信息,其中52例患者有配对的癌组织与癌旁组织。分析前列腺癌组织中CENPK基因mRNA表达水平,分析不同特征前列腺癌患者CENPK基因mRNA表达水平,比较CENPK基因高表达组与CENPK基因低表达组总体生存时间(OS)及无进展生存期(DFS),基因富集分析明确CENPK基因调控的相关信号通路。结果498例前列腺癌患者的癌组织和癌旁组织中CENPK基因mRNA的表达水平分别为3.9247±1.0929和3.4066±1.1545,差异有统计学意义(t=3.235,P=0.001)。52例配对的前列腺癌组织和癌旁组织中CENPK基因mRNA的表达水平分别为8.2071±0.2952和3.4066±1.1545,差异有统计学意义(t=28.309,P<0.001)。受试者工作特征(ROC)曲线分析显示CENPK基因可作为一个灵敏度和特异度较高的前列腺癌诊断指标(灵敏度为60.8%,特异度为63.5%,曲线下面积为0.625,95%CI为0.785~0.852)。有生化复发、PSA>4 ng/mL、Gleason评分>7分、有肿瘤残留、T分期为T3~T4期、有淋巴结转移的前列腺癌患者CENPK基因mRNA高表达率明显高于无生化复发、PSA≤4 ng/mL、Gleason评分≤7分、无肿瘤残留、T分期为T1~T2b期、无淋巴结转移的前列腺癌患者,差异有统计学意义(P<0.05)。CENPK基因高表达组OS及DFS明显短于CENPK基因低表达组,差异有统计学意义(P=0.045、0.003)。基因富集分析显示,CENPK基因可能通过碱基切除修复、核苷酸切除修复、基因复制、剪接体、错配修复等通路影响前列腺癌的发生、发展。结论CENPK基因在前列腺癌组织中呈高表达,且与临床特征及预后关系密切,可作为前列腺癌较好的诊断标志物。
Objective To investigate the expression and clinical significance of centromere protein K(CENPK)gene in prostate cancer.Methods By downloading relevant data from TCGA and GEPIA databases,mRNA expression level and clinical information of 498 patients with prostate cancer were obtained,among which 52 patients had paired cancer tissues and adjacent tissues.To analyze the mRNA expression levels of CENPK gene in prostate cancer tissues,analyze the mRNA expression levels of CENPK gene in prostate cancer patients with different characteristics,and compare the overall survival time(OS)and progression-free survival(DFS)between CENPK gene high expression group and CENPK gene low expression group.The signal pathways related to CENPK gene regulation were identified by gene enrichment analysis.Results The expression levels of CENPK gene mRNA in prostate cancer and adjacent tissues of the 498 prostate cancer samples were 3.9247±1.0929 and 3.4066±1.1545 respectively,the difference was statistically significant(t=3.235,P=0.001).The expression levels of CENPK gene mRNA in 52 matched prostate cancer and adjacent tissues were 8.2071±0.2952 and 3.4066±1.1545 respectively,the difference was statistically significant(t=28.309,P<0.001).Receiver operation characteristic(ROC)curve analysis indicated that CENPK gene could be used as a diagnostic index with high sensitivity and specificity for prostate cancer(sensitivity was 60.8%,specificity was 63.5%,AUC was 0.625,95%CI was 0.785-0.852).The high mRNA expression rates of CENPK gene in prostate cancer patients with biochemical recurrence,PSA>4 ng/mL,Gleason score>7,residual tumor,T stage T3-T4 and lymph node metastasis were significantly higher than those of prostate cancer patients with low biochemical recurrence,PSA≤4 ng/mL,Gleason score≤7,no tumor residue,T stage T1-T2b and no lymph node metastasis,with statistical significance(P<0.05).The OS and DFS of the CENPK high-expression group were significantly lower than those of the CENPK low-expression group,the differences were stat
作者
陆璐
谢光宇
冯耀宁
谢正飞
丁启健
谢智彬
LU Lu;XIE Guangyu;FENG Yaoning;XIE Zhengfei;DING Qijian;XIE Zhibin(Clinical School of Guilin Medical College,Guilin,Guangxi 541004,China;Department of Urology,the Fifth Affiliated Hospital of Guangxi Medical University,Nanning,Guangxi 530021,China)
出处
《检验医学与临床》
CAS
2023年第4期455-459,共5页
Laboratory Medicine and Clinic
基金
广西壮族自治区卫生健康委员会自筹经费科研课题(Z20191030、Z-A20221118)。
