摘要
目的:探讨类风湿关节炎(RA)患者血浆微小RNA(miRNA)-146a表达及基因高甲基化与疾病活动程度的关系。方法:2019年3月至2020年4月在我院临床免疫科招募122例RA患者为RA组,基于血细胞沉降率(ESR)获得28个关节疾病活动评分(DAS28-ESR)测量疾病活动性;另纳入同期健康志愿者141例为对照组。采用实时荧光定量PCR法检测血浆miR-146a水平,实时荧光定量甲基化特异性PCR(qMSP)分析甲基化状态。结果:RA组患者血浆miR-146a水平显著高于对照组,miR-146a基因甲基化水平显著低于对照组(P<0.05)。血浆miR-146a水平与miR-146a基因甲基化水平呈负相关(P<0.05)。随疾病活动程度的增加,RA患者血浆miR-146a水平呈升高趋势,miR-146a基因甲基化水平呈下降趋势(P<0.05)。血浆miR-146a水平与DAS28-ESR评分、ESR、C反应蛋白水平、关节肿胀计数、关节压痛计数、患者主观总体评估、医生总体评估、简化疾病活动指数呈正相关(P<0.05);miR-146a基因甲基化水平与上述指标呈负相关(P<0.05)。结论:血浆miR-146a及基因甲基化状态与RA患者疾病活动程度有关,有望作为诊断和控制RA疾病活动的潜在标志物。
Objective: To investigate the relationship between the plasma microRNA(miRNA)-146a expression, gene hypermethylation and the disease activity in patients with rheumatoid arthritis(RA). Methods: From March 2019 to April 2020, 122 RA patients were recruited into RA group in the rheumatology department of our hospital. The disease activity was measured using the 28-joint disease activity score obtained on the basis of erythrocyte sedimentation rate(ESR)(DAS28-ESR). During the same period, another 141 healthy volunteers were included as control group. The plasma miR-146a level was detected by real-time fluorescence quantitative PCR,and the methylation status was analyzed by real-time fluorescence quantitative methylation specific PCR(qMSP). Results: The plasma miR-146a level in the RA group was significantly higher than that in the control group, and the methylation level of miR-146a gene was significantly lower than that in the control group(P<0.05). Plasma miR-146a level was negatively correlated with miR-146a gene methylation level(P<0.05). With the increase of disease activity, the plasma level of miR-146a increased and the methylation level of miR-146a gene decreased(P<0.05). The plasma miR-146a level was positively correlated with DAS28-ESR, ESR, C-reactive protein, joint swelling count, joint tenderness count, patients’ subjective global assessment, physician’s global assessment and simplified disease activity index(P<0.05). The methylation level of miR-146a gene was negatively correlated with the above indexes(P<0.05). Conclusion: Plasma miR-146a and gene methylation status are related to the degree of disease activity in RA patients and may be potential markers for the diagnosis and control of RA disease activity.
作者
庞琳烜
杨西超
李治琴
杜望磊
PANG Linxuan;YANG Xichao;LI Zhiqin;DU Wanglei(Department of Clinical Immunology,the First Affiliated Hospital of Air Force Military Medical University,Xi’an 710032,China)
出处
《东南大学学报（医学版）》
CAS
2022年第6期818-824,共7页
Journal of Southeast University(Medical Science Edition)
基金
陕西省重点研发计划项目(2021SF-074)。
