摘要
目的探讨HLA-B、HLA-DR基因多态性与云南省金平县芒人三酰甘油血症及胆固醇血症的相关性。方法检测2020年8—12月收集的云南省金平县金水河镇的3个自然村中的202名自愿参与且16岁以上的芒人居民的血清三酰甘油(triglycerides,TG)及总胆固醇(total cholesterol,TC),采用寡聚核苷酸探针方法(PCR-SSO)检测其HLA-B、HLA-DR的基因型和血清型。结果芒人居民高TG的总患病率为26.7%,男性为28.4%,女性为25.0%;高TC的总患病率为13.9%,男性为12.7%,女性为15.0%。不同性别、年龄间患病率差异无统计学意义(P>0.05)。共检测出19种HLA-B位点等位基因、14种HLA-DRB1位点等位基因、12种HLA-B位点血清型、8种HLA-DRB1位点血清型。其中,HLA-B*40:01和HLA-DR*12:02是芒人的优势基因。高TG组HLA-B*13:01、HLA-B*40:02基因型及HLA-B*13血清型显著优于血脂正常组(13.5%vs 4.9%,0.0%vs5.7%,13.5%vs 4.9%),差异有统计学意义(χ^(2)=7.649,P=0.006;χ^(2)=4.339,P=0.037;χ^(2)=7.649,P=0.006);高TG组的血清型HLA-B*15显著低于正常组(3.1%vs 10.6%),差异有统计学意义(χ^(2)=4.931,P=0.026)。高TC组血清型HLA-B*15显著高于正常组(21.2%vs 10.6%),差异有统计学意义(χ^(2)=4.423,P=0.035)。结论携带HLA-B*13:01、HLA-B*40:02及HLA-B*13的芒人罹患高TG的风险显著高于未携带者,携带HLA-B*15的芒人罹患高TG的风险显著低于未携带者,罹患高TC的风险高于未携带者。
Objective To investigate the correlation between HLA-B and HLA-DR gene polymorphisms and triglycer‐ides and cholesterol in Muong in Jinping County,Yunnan Province.Methods Serum triglycerides(TG)and total cho‐lesterol(TC)were measured in 202 voluntarily participating Mang residents aged 16 years or older from three natural villages in Jinshuihe Township,Jinping County,Yunnan Province,collected from August to December 2020.The geno‐types and serotypes of HLA-B and HLA-DR were detected by oligonucleotide probe method(PCR-SSO).Results The overall prevalence of high TG was 26.7%in Muong,28.4%in men and 25.0%in women,and the overall preva‐lence of high TC was 13.9%,12.7%in men and 15.0%in women.There was no significant difference in prevalence between sexes and ages.A total of 19 HLA-B locus alleles,14 HLA-DRB1 locus alleles,12 HLA-B locus serotypes and 8 HLA-DRB1 locus serotypes were detected.Among them,HLA-B*40:01 and HLA-DR*12:02 were the domi‐nant genes in Muong.The HLA-B*13:01 and HLA-B*40:02 genotypes and HLA-B*13 serotypes were significantly better in the high-TG group than in the normal lipid group(13.5%vs 4.9%,0.0%vs 5.7%,13.5%vs 4.9%),the dif‐ference was statistically significant(χ^(2)=7.649,P=0.006;χ^(2)=4.339,P=0.037;χ^(2)=7.649,P=0.006).Serotype HLA-B*15 was significantly lower in the high TG group than in the normal group(3.1%vs 10.6%),the difference was statisti‐cally significant(χ^(2)=4.931,P=0.026).Serotype HLA-B*15 was significantly higher in the high TC group than in the normal group(21.2%vs 10.6%),the difference was statistically significant(χ^(2)=4.423,P=0.035).Conclusion The risk of developing high TG was significantly higher in Muong carrying HLA-B*13:01,HLA-B*40:02 and HLA-B*13 than in non-carriers.The risk of high TG was significantly lower in Muong carrying HLA-B*15 than in non-carriers,and the risk of high TC was higher than in non-carriers.
作者
黄娟
刘丽
何俊梅
白世光
李红
金兰花
刘宇
李久龙
金吕飞
HUANG Juan;LIU Li;HE Junmei;BAI Shiguang;LI Hong;JIN Lanhua;LIU Yu;LI Jiulong;JIN Lyufei(Department of Rheumatology and Immunology,the Fifth Affiliated Hospital of Kunming Medical University,Gejiu,Yunnan Province,661000 China;Department of Internal Medicine,Jinping County People's Hospital,Jinping,Yun-nan Province,661500 China)
出处
《系统医学》
2022年第22期1-5,共5页
Systems Medicine
基金
云南省教育厅科学研究基金项目(2021J032)。
