摘要
为探究载气对稻草秸秆固态发酵性能的影响特性,该文构建了一套新型气载乙醇同步酶解纤维素填充床发酵系统。在该系统中,将纤维素基质、纤维素酶及纤维二糖酶混合均匀,装填于反应器内形成基质填充床,酵母菌循环液流经床层将酶解发酵生成的葡萄糖转化为乙醇,利用载气将生成的产物及时载出反应器,乙醇被水吸收。结果表明:载气可及时将生成的产物载出反应器,提高发酵效率。载气类型对发酵过程影响较大,N2为载气时发酵效率最高,乙醇得率相对于无气提式发酵提高了1.04倍,间歇时间为2 h时乙醇得率最大,4 h乙醇总量最多为1.912 g;CO_(2)为载气时乙醇得率提高了0.94倍,间歇时间为4 h时乙醇得率最大且积累量最多,为1.974 g;然而空气为载气时对发酵效率提高不大,间歇时间为4 h时发酵效果最好,乙醇总量最多为1.592 g;最终结果表明载气为N2,间歇时间为2 h,乙醇得率最高。
In order to investigate the effect of carrier gas on the solid-state fermentation of rice straw,a new type of carrier gas-fed-batch fermentation system for simultaneous enzymatic hydrolysis of ethanol was constructed.In this system,the cellulose matrix,cellulase and cellobiose are mixed uniformly and filled into the reactor to form a matrix packed bed,the glucose produced by enzymatic hydrolysis and fermentation is converted into ethanol by yeast circulating liquid flowing through the bed,and the product is loaded into the reactor in time by carrier gas,and the ethanol is absorbed by water.The results showed that carrier gas could carry the product out of the reactor in time and improve the fermentation efficiency.The type of carrier gas has a great influence on the fermentation process.When the carrier gas was nitrogen,the fermentation efficiency was the highest,the ethanol yield was 1.04 times higher than that of non-air-lift fermentation,and the ethanol yield was the highest when the intermittent time was 2 hours,the maximum amount of ethanol in 4 hours was 1.912 g.The yield of ethanol increased 0.94 times when carbon dioxide was carrier gas,and the maximum amount of ethanol was 1.974 g when the interval time was 4 hours.However,when air was used as carrier gas,the fermentation efficiency was not improved,when the interval time was 4 hours,the best fermentation effect was achived and the maximum amount of ethanol was 1.592 g.The final results showed that when the carrier gas was N2and the intermittent time was 2 h,the yield of ethanol was the highest.
作者
李飞凤
董亮
刘自山
姚明材
罗广
LI Feifeng;DONG Liang;LIU Zishan;YAO Mingcai;LUO Guang(School of Bioengineering,Sichuan Light and Chemical Engineering University,Zigong 643000,China)
出处
《环境科学与技术》
CAS
CSCD
北大核心
2022年第11期165-173,共9页
Environmental Science & Technology
基金
酿酒生物技术及应用四川省重点实验室项目(NJ2021-05)
四川轻化工大学人才引进项目(2018RCL24)
四川轻化工大学大学生创新创业训练计划项目(cx2020200,Y2022080)
省级大创项目(S202210622077)。
关键词
纤维素乙醇载气
同步酶解发酵
固态发酵
cellulosic ethanol carrier gas
synchronous enzymolysis fermentation
solid state fermentation
