摘要
目的观察穴位埋线调控SIRT1/NF-κB信号通路对溃疡性结肠炎(ulcerative colitis,UC)大鼠炎症反应和肠黏膜屏障功能的影响。方法将Sprague Dawley(SD)大鼠随机分为空白组、模型组、药物组和穴位埋线组,每组10只。采用3%葡聚糖硫酸钠自由饮用建立UC大鼠模型。药物组给予柳氮磺胺吡啶每千克体质量0.5 g连续灌胃;穴位埋线组予穴位简易埋线治疗。比较4组大鼠疾病活动度指数(disease activity index,DAI)和黏膜损伤指数(colon mucosa damage index,CMDI)的改变,观察4组结肠组织的病理变化,比较血清和结肠组织髓过氧化物酶(myeloperoxidase,MPO)活性、结肠组织核因子-κB(nuclear factor-κB,NF-κB)p65的阳性表达、结肠组织SIRT1/NF-κB信号通路相关蛋白的表达、结肠组织黏膜屏障保护因子上皮钙黏附素(epithelial calcium-dependent cell adhesion molecule,E-cadherin)和紧密连接蛋白的闭合蛋白(occludin)的m RNA表达。结果与模型组比较,穴位埋线组大鼠DAI和CMDI评分降低,结肠黏膜溃疡和炎性浸润显著减轻,血清和结肠组织中MPO活性升高,结肠组织中NF-κB p65、p-NF-κB p65、p-IκBα、p-IKKβ、AC-p65的蛋白表达下调,SIRT1的蛋白表达水平及E-cadherin、occludin的m RNA水平上调,差异均具有统计学意义(P<0.05)。结论穴位埋线疗法可显著改善大鼠肠黏膜的炎症,降低DAI、CMDI及病理组织学评分,其作用机制可能与调控SIRT1/NF-κB信号通路、上调黏膜屏障保护因子水平及修复黏膜上皮屏障功能相关。
Objective To observe the effects of acupoint thread embedding on inflammatory reactions and intestinal mucosal barrier by regulating SIRT1/NF-κB signaling pathway in ulcerative colitis(UC)rats.Method Sprague Dawley(SD)rats were randomized into a blank control group,a model group,a medication group,and an acupoint thread embedding group,with 10 rats in each group.The UC rat model was established by drinking 3%Dextran sulfate sodium(DSS)ad libitum.Rats in the medication group were given Sulfasalazine by successive gastric gavage at 0.5 g for per kilogram body weight.The acupoint thread embedding group received a simple thread embedding intervention.Changes in the disease activity index(DAI)and colon mucosa damage index(CMDI)were compared among groups;pathological changes of colon tissues were observed;the activity of myeloperoxidase(MPO)in the serum and colon tissue,positive expression of colonic nuclear factor-κB(NF-κB)p65,expression of proteins associated with SIRT1/NF-κB signaling pathway in the colon tissue,and the m RNA expression of mucosal barrier protective factors epithelial calcium-dependent cell adhesion molecule(E-cadherin)and occludin in the colon tissue were also compared.Result Compared with the model group,the DAI and CMDI scores dropped in the acupoint thread embedding group,together with increased MPO activity in the serum and colon tissue,down-regulated protein expression of NF-κB p65,p-NF-κB p65,p-IκBα,p-IKKβ,and AC-p65 in the colon tissue,and up-regulated protein expression of SIRT1 and m RNA expression of E-cadherin and occludin,all presenting statistical significance(P<0.05).Conclusion Acupoint thread embedding can significantly improve inflammatory symptoms in the rat’s intestinal mucosa and reduce DAI,CMDI,and histopathological scores;the mechanism may be related to modulating SIRT1/NF-κB signaling pathway,up-regulating mucosal protective factor levels,and repairing the mucosal epithelial barrier function.
作者
李玎玎
刘朝霞
LI Dingding;LIU Zhaoxia(Heilongjiang University of Chinese Medicine,Harbin 150040,China;First Affiliated Hospital,Heilongjiang University of Chinese Medicine,Harbin 150040,China)
出处
《上海针灸杂志》
CSCD
2023年第2期182-189,共8页
Shanghai Journal of Acupuncture and Moxibustion
基金
黑龙江省中医药科研项目(ZHY-008)。
