摘要
血管紧张素转化酶(ACE)通过引起血管收缩,在高血压中起关键作用,抑制ACE的活性对降血压有积极影响。乳酸菌在发酵牛乳过程中可水解蛋白产生ACE抑制肽,从而产生降压效果。本文利用前期从健康母乳中分离的益生菌——植物乳杆菌B联合酸奶发酵剂制备水牛乳酸奶,通过紫外分光光度法和液相质谱联用系统(LC-MS/MS)分析了水牛乳酸奶ACE抑制活性和ACE抑制肽分布特征。结果表明,添加植物乳杆菌B的水牛乳酸奶的ACE抑制率为84.51%,经10 k、3 k超滤膜依次处理后,ACE抑制活性进一步提高,分别达到88.24%、92.38%。肽鉴定结果表明,含植物乳杆菌B的水牛乳酸奶发酵过程中产生了9种已知ACE抑制肽(RFFVAPFPEVFGK、FVAPFPEVFGK、PIGSENSGKTTMPLW、NMAINPSKE、RELEELNVPGEIVE、DELQDKIHPF、ELQDKIHPF、DKIHPFAQ、SQSKVLPVPQ)和许多潜在的ACE抑制肽。其中来源于β-酪蛋白的21肽EMPFPKYPVEPFTESQSLTLT(f123-143)及23肽HKEMPFPKYPVEPFTESQSLTLT(f121-143)含有已知ACE抑制肽EMPFPK、EMPFPKYPVEPF、TESQSLTLT和HKEMPFPKYPVEPF,推测该多肽具有较强的降血压功能,其ACE抑制作用和机制值得进一步研究。本研究结果为开发降血压益生菌水牛乳产品及其功效的深入研究奠定了基础。
Angiotensin-converting enzyme(ACE)plays a key role in hypertension by causing vasoconstriction,and inhibiting the activity of ACE has a positive effect on lowering blood pressure. In the process of fermenting milk,lactic acid bacteria can hydrolyze proteins to produce ACE inhibitory peptides,thereby producing an antihypertensive effect. In this paper,buffalo milk yogurt was prepared by using Lactobacillus plantarum B,a probiotic isolated from healthy breast milk,combined with yogurt starter. The ACE inhibitory activity of buffalo milk yogurt and the distribution characteristics of ACE inhibitory peptides were analyzed by UV spectrophotometry and liquid chromatography-mass spectrometry(LC-MS/MS). The results showed that the ACE inhibition rate of buffalo lactic acid milk added with Lactobacillus plantarum B was 84.51%. After treatment with 10 K and 3 K ultrafiltration membrane,the ACE inhibition activity was further improved,reaching 88.24% and 92.38% respectively. The results of peptide identification showed that nine known ACE inhibitory peptides(RFFVAPFPEVFGK,FVAPFPEVFGK,PIGSENSGKTTMPLW,NMAINPSKE,RELEELNVPGEIVE,DELQDKIHPF,ELQDKIHPF,DKIHPFAQ and SQSKVLPVPQ)and many potential ACE inhibitory peptides were produced during the fermentation of buffalo lactic acid milk containing Lactobacillus plantarum B. Among them,21 peptides EMPFPKYPVEPFTESQSLTLT(f123-143)and 23 peptides HKEMPFPKYPVEPFTESQSLTLT(f121-143)derived from β-casein contain known ACEinhibiting peptides EMPFPK,EMPFPKYPEPF,TESQSLTLT and HKEMPFPKYPVEPF,It is speculated that the polypeptide has strong antihypertensive function. Its ACE inhibitory effect and mechanism deserve further study,and the results of this study lay a foundation for the development of blood pressure-lowering probiotic buffalo milk products and their efficacy.
作者
贺小龙
姜铁民
陈历俊
秦颖
张欣
蒋菲燕
陈振宇
HE Xiaolong;JIANG Tiemin;CHEN Lijun;QIN Ying;ZHANG Xin;JIANG Feiyan;CHEN Zhenyu(South Asia Branch of National Engineering Center of Dairy for Maternal and Child Health,Guilin University of Technology,Guilin 541004;Beijing Sanyuan Foods Co.,Ltd.,Beijing 100163)
出处
《中国食品添加剂》
CAS
北大核心
2023年第2期35-42,共8页
China Food Additives
基金
广西科技计划项目(AD20297088)
中央引导地方科技发展专项资金(桂科ZY22096025)
桂林理工大学大学生创新创业项目(202110596023)。
关键词
ACE抑制肽
母乳益生菌
植物乳杆菌
水牛乳
ACE inhibitory peptide
breast milk probiotics
Lactobacillus plantarum
buffalo milk
