摘要
目的探讨在放射性膀胱炎模型中淫羊藿次苷Ⅱ(ICAⅡ)对膀胱的保护作用和机制。方法2021年7月至2022年3月取10周龄SD雄性大鼠18只,采用随机数字表法分为对照组、模型组、治疗组,每组各6例。模型组和治疗组予单一剂量为20 Gy的X线盆腔区域辐照,照射后24 h,治疗组予ICAⅡ4.5 mg/(kg·d)灌胃,对照组和模型组予相同体积的溶剂(10%无水乙醇、20%异丙醇、30%聚乙二醇和40%去离子水的混合物)灌胃,连续4周,药物洗脱1周。采用多导电生理仪检测3组大鼠的膀胱容量和膀胱漏尿点压力,评估膀胱功能。取3组大鼠的膀胱标本行HE染色、Masson染色、ELISA、TUNEL法和蛋白质印迹法检测,比较3组大鼠膀胱组织的病理变化(膀胱黏膜厚度、平滑肌与胶原纤维的比值)、氧化应激水平(超氧化物歧化酶SOD、丙二醛)、细胞凋亡率、炎症因子(IL-6、NF-kB)和抗氧化应激信号通路因子(Nrf2、HO-1)蛋白表达量的变化。结果建模4周后,模型组的膀胱容量[(1.01±0.12)ml与(1.58±0.21)ml,P=0.001]、膀胱漏尿点压力[(38.79±4.12)cmH2O(1 cmH2O=0.098 kPa)与(60.59±3.81)cmH2O,P=0.001]、膀胱壁平滑肌与胶原纤维比值[1.78±0.17与3.15±0.57,P=0.001]、SOD[(6.31±0.73)U/mg与(14.67±1.04)U/mg,P=0.001]低于对照组,差异均有统计学意义。模型组的膀胱黏膜厚度[(47.33±1.78)μm与(20.83±2.33)μm,P=0.001]、丙二醛[(1.01±0.13)nmol/mg与(0.49±0.03)nmol/mg,P=0.001]、IL-6(0.87±0.11与0.33±0.10,P=0.001)、NF-kB(0.71±0.14与0.29±0.07,P=0.001)、细胞凋亡率[(11.60±3.04)%与(3.91±1.40)%,P=0.007]高于对照组,差异均有统计学意义。治疗组的膀胱容量[(1.27±0.13)ml,P=0.030]、膀胱漏尿点压力[(47.83±2.50)cmH2O,P=0.004]、平滑肌与胶原纤维比值(2.78±0.68,P=0.015)、SOD[(10.48±0.85)U/mg,P=0.001]高于模型组,膀胱黏膜厚度[(31.94±3.20)μm,P=0.001]、丙二醛[(0.64±0.09)nmol/mg,P=0.001]、IL-6(0.69±0.11,P=0.035)、NF-kB(0.45±0.06,P=0.002)、细胞凋亡率[
Objective To investigate the protective effect and mechanism of IcaritinⅡ(ICAⅡ)on bladder in radiation cystitis model.Methods A total of 1810-week-old male SD rats were selected from July 2021 to March 2022 and divided into control group,model group and treatment group by random number table method,with 6 cases in each group.Model group and treatment group were given a single dose of 20 Gy X-ray irradiation in the pelvic area.24 h after irradiation,the treatment group was given ICAⅡ4.5 mg/(kg·d)gavage,while the control group and model group were given the same volume of solvent(10%anhydrous ethanol,20%isopropyl alcohol,30%polyethylene glycol and 40%deionized water)gavage for 4 consecutive weeks.Drug eluting for 1 week.The bladder volume and leakage point pressure of the three groups of rats were measured by multi-conducting physiological apparatus,and the bladder function was evaluated.HE staining,Masson staining,ELISA,TUNEL staining and western blotting were performed on the bladder samples of the three groups of rats.The pathological changes(thickness of bladder mucosa,ratio of smooth muscle to collagen fiber),oxidative stress level(superoxide dismutase SOD,malondialdehyde),apoptosis rate,protein levels of inflammatory factors(IL-6,NF-kB)and anti-oxidative stress signaling pathway factors(Nrf2,HO-1)of the three groups of rats were compared.Results After 4 weeks of modeling,in the model group,the bladder volume[(1.01±0.12)ml vs.(1.58±0.21)ml,P=0.001],the bladder leakage point pressure[(38.79±4.12)cmH2O(1 cmH2O=0.098 kPa)vs.(60.59±3.81)cmH2O,P=0.001],the ratio of smooth muscle of bladder wall to collagen fiber[1.78±0.17 vs.3.15±0.57,P=0.001],SOD[(6.31±0.73)U/mg vs.(14.67±1.04)U/mg,P=0.001]were lower than the control group,and the differences were statistically significant.In the model group,the thickness of bladder mucosa[(47.33±1.78)μm vs.(20.83±2.33)μm,=,P=0.001],malondialdehyde[(1.01±0.13)nmol/mg vs.(0.49±0.03)nmol/mg,P=0.001],IL-6(0.87±0.11 vs.0.33±0.10,P=0.001),NF-kB(0.71±0.14 vs.0.29
作者
孙吉磊
许永德
魏志涛
刘洋
刘树坤
王明星
杨勇
Sun Jilei;Xu Yongde;Wei Zhitao;Liu Yang;Liu Shukun;Wang Mingxing;Yang Yong(Department of Urology,The Affiliated Hospital of Changchun University of Chinese Medicine,Changchun 130021,China;Department of Urology,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China;Laboratory,The Affiliated Hospital of Changchun University of Chinese Medicine,Changchun 130021,China)
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
2022年第12期929-935,共7页
Chinese Journal of Urology
