摘要
【目的】测定番茄红素胶束中番茄红素Z/E异构体含量。【方法】采用HPLC法,色谱柱为YMC Carotenoid S-5(4.6 mm×250 mm);流动相A:乙腈B:甲基叔丁基醚;线性梯度洗脱;流速:1 mL/min;检测波长:472 nm;柱温:30℃,在45 min内完成异构体的分离检测,通过紫外-可见吸收光谱图上的特征峰及吸收强度,分析和鉴定番茄红素胶束中存在的异构体。【结果】番茄红素在0.22~6.57μg/mL线性关系良好(r=0.999);加样回收率为100.06%(RSD=1.99%),该方法专属性强,线性关系良好,快速、准确、简便,适用于番茄红素胶束中异构体的含量测定。【结论】番茄红素胶束中存在全反式:All-E,以及三个顺式异构体:13 Z,9 Z,5 Z,其平均相对百分含量分别为76.06%、10.83%、4.55%、8.56%。
【Objective】To determine the content of Z/E isomers in lycopene micelles.【Method】HPLC method,The column was YMC Carotenoid S-5(4.6 mm×250 mm);Mobile phase A:acetonitrile B:methyl tert-butyl ether;Linear gradient elution;Flow rate:1 mL/min;Detection wavelength:472 nm;Column temperature:30℃,Complete the separation and detection of isomers within 45 minutes,the isomers in the lycopene micelles are analyzed and identified through the characteristic peaks and absorption intensity on the ultraviolet-visible absorption spectrum.【Result】The linear ranges of Lycopene was 0.22~6.57 μg/mL(r=0.999),Whose average recoveries were 100.06%(RSD=1.99%). It shows that the method has strong specificity,good linear relationship,fast,accurate and simple,and is suitable for the determination of isomers in lycopene micelles.【Conclusion】Determined that there are all-trans forms in the lycopene micelles:All-E,and three cis isomers:13Z,9Z,5Z.with an average relative percentage of 76.06%,10.83%,4.55%,8.56%,respectively.
作者
马雪红
刘婷
李传天
朱金芳
MA Xuehong;LIU Ting;LI Chuantian;ZHU Jinfang(College of Food Science and Pharmaceutical Science,Xinjiang Agricultural University,Urumqi 830052,China)
出处
《新疆农业科学》
CAS
CSCD
北大核心
2022年第9期2209-2216,共8页
Xinjiang Agricultural Sciences
基金
国家自然科学基金(31960476)。
关键词
番茄红素
胶束
异构体
含量测定
lycopene
micelles
isomers
content determination
