摘要
巨型囊泡不仅拥有类似于生物细胞质膜的双分子层闭合结构,而且具有类似细胞大小的尺寸,因而被广泛应用于生物膜功能模拟。巨型囊泡很容易通过光学显微成像系统进行观察,使其在可视化传感分析方面具有广阔的应用前景。本文重点介绍了巨型囊泡的常用制备方法,如膜水化法、乳液模板法、聚合诱导自组装方法等,总结了近年来巨型囊泡用于生物模拟尤其是在成像分析方面的应用,并对其未来研究方向进行了展望。
Giant vesicles not only possess a bilayer closure structure similar to the plasma membrane of biological cells,but also have cell-like size,and thus are widely used in the simulation of biological membrane functions.Meanwhile,giant vesicles are easily observed by optical microscopy imaging systems,affording broad application prospects in visual sensing analysis.In this review,we focused on the common methods for preparing giant vesicles(such as film hydration,emulsion template,polymerization-induced self-assembly methods,etc.),summarized the recent applications of giant vesicles in biological simulation(especially in imaging analysis),and provided an outlook on future research directions in this field.
作者
肖秀
唐晓芳
周成成
管伟江
XIAO Xiu;TANG Xiaofang;ZHOU Chengcheng;GUAN Weijiang(State Key Laboratory of Chemical Resource Engineering,Beijing University of Chemical Technology,Beijing 100029,China;Green Catalysis Center,College of Chemistry,Zhengzhou University,Zhengzhou 450001,China;School of Chemistry and Chemical Engineering,Yangzhou University,Yangzhou 225002,China)
出处
《分析试验室》
EI
CAS
CSCD
北大核心
2022年第12期1425-1434,共10页
Chinese Journal of Analysis Laboratory
基金
北京市自然科学基金(2212013)
国家自然科学基金(21804006,22002137)项目资助。
关键词
巨型囊泡
人工细胞
自组装
生物模拟
成像分析
giant vesicles
artificial cells
self-assembly
biomimicry
imaging analysis
