摘要
【背景】七味白术散可用于治疗气阴两虚所致的消渴病,尤其近些年来在改善糖尿病胰岛素抵抗方面得到广泛使用。【目的】观察七味白术散对糖尿病大鼠肠道屏障和炎症因子的影响,并探讨其作用机制。【方法】60只无特定病原体(specific pathogen free,SPF)级SD雄性大鼠随机分为空白组、模型组、二甲双胍组和七味白术散高、中、低剂量组,每组10只。腹腔注射链脲佐菌素(streptozotocin,STZ)的方法建立糖尿病大鼠模型,空白组大鼠给予正常饮食,模型组、二甲双胍组和七味白术散高、中、低剂量组给予高糖高脂饮食。造模成功后分别用二甲双胍和七味白术散灌胃处理,空白组和模型组生理盐水灌胃。观察治疗前后大鼠的一般生存状况;药物干预4周后,全自动生化分析仪检测空腹血糖(fasting blood glucose,FBG)、空腹胰岛素(fasting insulin,FINS)、脂多糖(lipopolysaccharide,LPS);酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)测定血清白细胞介素-6(interleukin-6,IL-6)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)水平;采用苏木精-伊红(hematoxylin-eosin,HE)染色法观察各组大鼠结肠组织病理改变,免疫组化法观察结肠组织occludin蛋白的表达位置及表达水平,Western blotting法检测回肠组织中zonula occludens-1(ZO-1)、occludin、Toll-like receptor 4(TLR4)、核因子kappa B(nuclear factor kappa B,NF-κB)蛋白的表达。【结果】与空白组比较,造模后的各组大鼠空腹血糖值≥11.1 mmol/L,差异有统计学意义(P<0.01)。与模型组比较,七味白术散低、中剂量组大鼠FBG有所下降,差异无统计学意义(P>0.05);七味白术散高剂量组和二甲双胍组大鼠FBG均明显下降,与模型组比较差异有统计学意义(P<0.05)。与模型组比较,七味白术散低、中、高剂量组和二甲双胍组大鼠FINS、LPS、IL-6、TNF-α水平均显著降低,差异有统计学意义(P<0.01)。与空白组比较,�
[Background]Qiwei Baizhu San can be used to treat diabetes caused by deficiency of both qi and yin.Especially in recent years,it has been widely used in improving insulin resistance of diabetes mellitus.[Objective]To observe the effects of Qiwei Baizhu San on intestinal barrier and inflammatory factors in diabetic rats and to explore its mechanism.[Methods]Sixty SD male rats in specific pathogen free(SPF)grade were randomly divided into a normal control group,a model group,a metformin group,and high,medium,and low-dose Qiwei Baizhu San groups,with 10 rats in each group.The diabetic rat model was established by intraperitoneal injection of streptozotocin(STZ).Rats in the normal control group were given a normal diet,and rats in the model group,the metformin group,and the high,medium,and low-dose Qiwei Baizhu San groups were all given high-sugar and high-fat diets.After successful modeling,the metformin group and the Qiwei Baizhu San groups were treated with metformin and Qiwei Baizhu San by gavage,respectively.Normal saline was given by gavage in the normal control group and the model group.The general living status of rats before and after treatment was observed.After 4 weeks of drug intervention,fasting blood glucose(FBG),fasting insulin(FINS),and lipopolysaccharide(LPS)were detected by an automatic biochemical analyzer.Interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)in rat serum were measured by enzyme-linked immunosorbent assay(ELISA).Hematoxylin-eosin(HE)staining was used to observe the pathological changes in colon tissues of rats in each group,and immunohistochemistry was used to observe the expression position and level of occludin protein in colon tissues.Western blotting assay was used to determine the protein expressions of zonula occludens-1(ZO-1),occludin,Toll-like receptor 4(TLR4),and nuclear factor kappa B(NF-κB).[Results]As compared with the normal control group,the FBG of rats after modeling in each group was≥11.1 mmol/L(P<0.01).As compared with the model group,the FBG of rats in the low
作者
王翠菊
苗秀明
郭良清
韩晓春
柳琳
钱宇洁
张秀婷
WANG Cuiju;MIAO Xiuming;GUO Liangqing;HAN Xiaochun;LIU Lin;QIAN Yujie;ZHANG Xiuting(The First Clinical Medical College,Shandong University of Traditional Chinese Medicine,Ji՚nan 250014,Shandong,China;Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Ji՚nan 250014,Shandong,China;Health College of Shandong University of Traditional Chinese Medicine,Ji՚nan 250014,Shandong,China;School of Traditional Chinese Medicine,Shandong University of Traditional Chinese Medicine,Ji՚nan 250014,Shandong,China)
出处
《微生物学通报》
CAS
CSCD
北大核心
2023年第1期313-323,共11页
Microbiology China
基金
国家自然科学基金(81703838,81704038)
山东省自然科学基金(ZR2019MH064)
山东省重点研发计划(2019GSF108210)
山东省中医药科技发展计划(2019-0169,2015078)。
