摘要
利用基因干扰技术研究转录因子PDIDSM_85260在指状青霉生长过程中的生理功能。采用菌丝生长和孢子萌发的方法研究沉默基因PDIDSM_85260对指状青霉在正常和胁迫条件下生长特性的影响。结果表明:基因PDIDSM_85260对在正常生长条件下指状青霉的孢子萌发和菌丝形态影响较小,但会影响指状青霉的生长;与野生型菌株相比,沉默基因PDIDSM_85260对胞外K^(+)、Na^(+)、Fe^(2+)和Ca^(2+)等金属离子和H_(2)O_(2)的敏感性增加,说明转录因子PDIDSM_85260在指状青霉的生长过程中以及在维持细胞离子稳态、活性氧水平和氧化应激方面发挥了重要的作用。本研究为进一步研究指状青霉转录因子PDIDSM_85260的功能奠定了基础。
The aim of this study was to study the physiological function of PDIDSM_85260 in the growth of Penicillium digitatum.The effect of silencing of gene PDIDSM_85260 was investigated on the growth characteristics of Penicillium digitatum under normal and stress conditions by measuring mycelial growth and spore germination.Microscopy characterization showed that PDIDSM_85260 silencing had no effect on spore germination and mycelial morphology under normal growth conditions.However,the silence of the gene PDIDSM_85260 leaded to the increase of growth rate of Penicillium digitatum.In addition,the responses of Penicillium digitatum upon ions(Na^(+),K^(+),Mg^(2+),Cu^(2+),Fe^(2+),Ca^(2+))and H_(2)O_(2) stress were also evaluated.Compared with the wild-type strain,silencing of the PDIDSM_85260 gene increased the sensitivity to the stress of extracellular K^(+),Na^(+),Fe^(2+),Ca^(2+),demonstrating that PDIDSM_85260 may be involved in the response of Penicillium digitatum to ions stress.Also,the high concentrations of H_(2)O_(2) significantly inhibited the growth of Penicillium digitatum,and PDIDSM_85260 silencing increased the sensitivity to the stress of H_(2)O_(2).Therefore,PDIDSM_85260 may participate in the maintenance of reactive oxygen species and oxidative stress.In summary,this study suggested that the transcription factor PDIDSM_85260 may play an important role in the growth of Penicillium digitatum,maintenance of ionic homeostasis,resistance to reactive oxygen species and oxidative stress.This study provided a foundation for further study on the function of PDIDSM_85260 in Penicillium digitatum.
作者
陈燕
赵仁勇
张璐璐
CHEN Yan;ZHAO Renyong;ZHANG Lulu(College of Food Science and Engineering,Henan University of Technology,Zhengzhou 450001,China)
出处
《河南工业大学学报（自然科学版）》
CAS
北大核心
2022年第6期37-44,52,共9页
Journal of Henan University of Technology：Natural Science Edition
基金
国家自然科学基金青年科学基金项目(32101868)
河南工业大学高层次人才基金项目(2021BS054)。
