摘要
目的探讨外周血富含亮氨酸的Alpha-2糖蛋白-1(LRG1)和缺氧诱导因子1α(HIF-1α)联合核因子-红细胞-2相关因子2(Nrf-2)蛋白对肛瘘镜下手术治疗肛瘘患者切口感染的早期诊断价值。方法回顾性选取2019年6月至2021年12月在秦皇岛市第一医院肛肠外科接受肛瘘镜下手术治疗肛瘘的126例患者作为研究对象,根据是否发生术后切口感染分为感染组(n=20)和非感染组(n=106)。采集术前、术后第1天和术后第3天外周静脉血,酶联免疫吸附试验分析血清LRG1、HIF-1α和Nrf-2水平,并采用操作者工作特征(ROC)曲线分析血清LRG1、HIF-1α和Nrf-2水平对术后切口感染发生的预测价值。结果感染组术后第1天和第3天血清LRG1、HIF-1α和Nrf-2水平均高于术前,且术后第1天高于术后第3天,差异均有统计学意义(P<0.05);非感染组术后第1天血清Nrf-2水平高于术前及术后第3天,差异均有统计学意义(P<0.05);术后第1天和第3天,感染组血清LRG1、HIF-1α和Nrf-2水平分别为(23.58±2.40)pg/mL、(85.12±14.06)pg/mL、(236.58±32.74)ng/mL和(15.09±2.85)pg/mL、(55.07±11.38)pg/mL、(186.19±27.04)ng/mL,均高于非感染组[第1天:(5.18±1.34)pg/mL、(44.89±7.30)pg/mL、(30.70±4.57)ng/mL;第3天:(5.04±1.12)pg/mL、(45.32±7.25)pg/mL、(28.56±4.72)ng/mL],差异均有统计学意义(P<0.05)。术后第1天血清LRG1、HIF-1α和Nrf-2水平单独及联合预测评估术后切口感染发生的ROC曲线下面积(95%CI)分别为0.805(0.642~0.971)、0.750(0.655~0.829)、0.763(0.592~0.946)、0.874(0.791~0.940)。结论肛瘘镜下手术治疗肛瘘患者有术后切口感染风险,术后第1天血清LRG1、HIF-1α和Nrf-2水平对术后切口感染的发生有一定预测价值,3者联合检测的预测价值更高。
Objective To investigate the levels of leucine-rich Alpha-2 Glycoprotein-1(LRG1)and hypoxia-inducible factor 1α(HIF-1α)in peripheral blood combined with nuclear factor-erythroid-2-related factor 2(Nrf2)protein in the early diagnosis of incision infection in patients with anal fistula treated under endoscopy.Methods From June 2019 to December 2021,126 patients who received anal fistula surgery under the anal fistula endoscope in Department of Anorectal Surgery the First Hospital of Qinhuangdao were retrospectively selected as the study subjects.They were divided into infection group(n=20)and non infection group(n=106)according to whether there was postoperative incision infection.Peripheral blood was collected before operation,the first day after operation and the third day after operation,and the levels of serum LRG1,HIF-1αand Nrf-2 were detected by enzyme-linked immunosorbent assay.The predictive value of serum LRG1,HIF-1αand Nrf-2 levels on the occurrence of postoperative incision infection was analyzed using the receiver operating characteristic(ROC)curve.Results The levels of serum LRG1,HIF-1αand Nrf-2 on the first and third days after operation in the infection group were higher than those before operation,the level of serum serum LRG1,HIF-1αand Nrf-2 on the first day after operation in the infected group were higher than that on the third day after operation,the differences were statistically significant(P<0.05);the level of serum Nrf-2 on the first day after operation in the non infected group was significantly higher than that before operation and on the third day after operation,the difference was statistically significant(P<0.05);the levels of serum LRG1,HIF-1αand Nrf-2 in the infection group on the first and third days after operation were(23.58±2.40)pg/mL,(85.12±14.06)pg/mL,(236.58±32.74)ng/mL and(15.09±2.85)pg/mL,(55.07±11.38)pg/mL,(186.19±27.04)ng/mL,respectively,which were higher than those in the non infected group[the first day:(5.18±1.34)pg/mL,(44.89±7.30)pg/mL,(30.70±4.57)ng/mL;
作者
夏长河
刘芳
张傲
夏长江
牛志新
闫丽丽
吴剑冬
XIA Chang-he;LIU Fang;ZHANG Ao(Department of Anorectal Surgery,the First Hospital of Qinhuangdao,Qinhuangdao Hebei 066000,China;Physical Examination Center,the First Hospital of Qinhuangdao,Qinhuangdao Hebei 066000,China)
出处
《临床和实验医学杂志》
2022年第23期2529-2532,共4页
Journal of Clinical and Experimental Medicine
基金
河北省卫生健康委医学科学研究计划项目(编号:20191387)
秦皇岛市科学技术研究与发展计划项目(编号:201902A171)。
