摘要
[Objectives]The paper was to establish in vitro propagation of an excellent germplasm of Codonopsis pilosula"Fengdang".[Methods]The effects of different plant growth regulators on callus induction and redifferentiation were tested by using sterile stem explants in vitro that germinated from seeds of superior"Fengdang"plant.[Results]The optimum medium for callus induction was MS+0.5 mg/L NAA+1.0 mg/L 16-BA.The optimal medium for bud differentiation was MS+0.1 mg/L NAA+0.5 mg/L 6-BA,and that for rooting was 1/2 MS+0.2 mg/L NAA+0.2 mg/L IBA.By using the media mentioned above,the rates of callus induction,bud differentiation and rooting reached 91%,100%and 97%,respectively.[Conclusions]The rapid propagation system of regenerated plants in vitro established in this study lays a foundation for the popularization and industrialized seedling of excellent germplasm of"Fengdang".
