摘要
目的探讨Garcinone C对鼻咽癌细胞衰老、迁移和侵袭功能的影响及作用机制。方法Garcinone C处理鼻咽癌细胞HONE1,通过β-半乳糖苷酶染色检测衰老细胞。应用Transwell实验检测Garcinone C干预对鼻咽癌细胞HONE1、HK1迁移、侵袭能力的影响。应用Western blot实验检测鼻咽癌细胞HONE1、HK1上皮间充质转化(EMT)标志蛋白E-钙黏蛋白(E-cadherin)、N-钙黏蛋白(N-cadherin)、金属蛋白酶组织抑制剂2(TIMP2)蛋白表达水平,以及铁死亡相关蛋白铁蛋白重链1(FTH1)、胱氨酸转运体蛋白xCT的表达水平。结果经Garcinone C处理后,HONE1细胞衰老率上升(P<0.05),HK1和HONE1细胞迁移和侵袭能力下降(P<0.05)。Garcinone C干预可上调HONE1、HK1细胞E-cadherin和TIMP2蛋白的表达水平(P<0.05),下调N-cadherin和FTH1、xCT蛋白的表达水平(P<0.05)。结论Garcinone C可能通过抑制鼻咽癌细胞的迁移、侵袭,并诱导其衰老和发生铁死亡的途径发挥治疗鼻咽癌的作用,具有应用的前景。
Objective To investigate the effects of Garcinone C on senescence,migration and invasion of nasopharyngeal carcinoma cells and their mechanisms of action.Methods Nasopharyngeal carcinoma cells HONE1 were treated with Garcinone C and the senescent cells were detected byβ-galactosidase staining.Transwell assay was used to detect the effects of Garcinone C intervention on the migration and invasion ability of nasopharyngeal carcinoma cells HONE1 and HK1.The expression levels of epithelial-mesenchymal transition(EMT)markers E-cadherin,N-cadherin and tissue inhibitor of matrix metalloproteinase 2(TIMP2),and the expression levels of iron death associated protein ferritin heavy chain 1(FTH1)and cystine transporter protein xCT in the nasopharyngeal carcinoma cells HONE1 and HK1 were detected by Western blot assay.Results After the treatment of Garcinone C,the senescence rate of HONE1 cells increased(P<0.05),and the migration and invasion ability of HK1 and HONE1 cells decreased(P<0.05).Garcinone C intervention could up-regulate the expression levels of E-cadherin and TIMP2 proteins in HONE1 and HK1 cells(P<0.05),and could down-regulate the expression levels of N-cadherin,FTH1 and xCT proteins(P<0.05).Conclusion Garcinone C may play a role in the treatment of nasopharyngeal carcinoma by inhibiting the migration and invasion of nasopharyngeal carcinoma cells and inducing their senescence and iron death,which has a promising application prospect.
作者
张菡
韦丹
蔡美婷
尹富强
刘夏
ZHANG Han;WEI Dan;CAI Mei-ting(Key Laboratory of Longevity and Aging-related Diseases of Chinese Ministry of Education,Center for Translational Medicine,Institute of Neuroscience and Guangxi Key Laboratory of Brain Science,Guangxi Medical University,Nanning 530021,China)
出处
《中国临床新医学》
2023年第1期25-30,共6页
CHINESE JOURNAL OF NEW CLINICAL MEDICINE
基金
国家自然科学基金地区科学基金项目(编号:82260721,81660606)
国家自然科学基金青年科学基金项目(编号:81903644)
广西自然科学基金面上项目(编号:2018GXNSFAA281227)。
关键词
Garcinone
C
鼻咽癌
迁移
侵袭
衰老
铁死亡
Garcinone C
Nasopharyngeal carcinoma
Migration
Invasion
Senescence
Ferroptosis
