摘要
肺类器官主要是由干细胞或特异性祖细胞通过3D培养形成的一种能够更加真实地在体外条件下模拟肺的结构和功能的组织类似物,与人体肺组织具有较高的组织源性。随着肺类器官技术的发展,其培养方案林立,但缺乏统一的标准。不过目前已经在疾病研究、药物筛选以及毒理评价等多个领域开展了肺类器官的相关研究。该文主要综述了肺类器官的培养技术及相关应用,以期为肺类器官的发展提供一定参考。
Lung organoids are mainly formed by stem cells or specific progenitor cells through 3D culture,which can simulate the structure and functions of lung in vitro,and have high tissue origin with human lung tissue.With the development of lung organoids,there are many culture methods,but there is no unified standard.However,studies on lung organoids have been carried out in many fields such as disease research,drug screening and toxicological evaluation.This paper mainly reviews the cultures and applications of lung organoids,in order to provide a certain reference for the development of lung organoids.
作者
王祥龙
田雨闪
陈欢
侯宏卫
胡清源
WANG Xianglong;TIAN Yushan;CHEN Huan;HOU Hongwei;HU Qingyuan(Key Laboratory of Tobacco Biological Effects,China National Tobacco Quality Supervision and Test Center Zhengzhou 450001,China)
出处
《中国细胞生物学学报》
CAS
CSCD
2022年第10期2006-2012,共7页
Chinese Journal of Cell Biology
基金
国家烟草质量监督检验中心青年人才托举工程项目(批准号:552021CR0030)
环境化学与生态毒理学国家重点实验室开放基金(批准号:KF2021-17)
中国烟草总公司科技重大专项(批准号:110202101018(XX-04))资助的课题。
关键词
肺类器官
肺类器官的培养
肺类器官的应用
lung organoids
lung organoids cultures
lung organoids applications
