摘要
Advances in single-cell sequencing technologies have made it possible to reveal the cellular basis of complex organ regeneration processes.In this review,we briefly introduce commonly used high-throughput single-cell sequencing platforms and their characteristics.We discuss in depth how single-cell sequencing techniques can be used in the study of regeneration to reveal stem/progenitor cell types and their developmental trajectories.From the perspective of stem cells,we review animal models of regenerative biology and organ models involved in regenerative medicine,with a focus on limb/fin/digit-tip regeneration.Finally,we discuss previous prospective work on improving systematic approaches to explore the origin and evolution of cellular diversity,as well as future research directions.
基金
supported by grants from the National Natural Science Foundation of China(32270489).
