摘要
为提高裂褶菌胞内多糖得率,并研究裂褶菌多糖纯化组分的生物活性,本研究采取超声波破碎和热水浸提相结合的方法提取裂褶菌胞内粗多糖,利用响应面法对提取条件进行优化,通过DEAE-52离子交换柱层析和Sephadex G-100凝胶柱层析对提取的粗多糖进行分离纯化,并进行结构表征、抗氧化性和抑菌性试验。结果表明,裂褶菌胞内粗多糖的最优提取条件为:提取温度90℃,水料比30:1,超声时间30 min,超声功率230 W,裂褶菌胞内粗多糖得率达到了18.14%±0.33%。纯化多糖组分NSPG-1为β型吡喃糖,分子量为1.05×10^(6) Da,NSPG-1多糖具有较好的抗氧化性和抑菌性,其对DPPH自由基、羟基自由基和超氧阴离子自由基的IC_(50)值分别为6.97、1.07和11.41 mg/mL,对大肠杆菌、枯草芽孢杆菌和金黄色葡萄球菌的IC_(50)值分别为7.56、12.54和10.42 mg/mL。本研究结果为裂褶菌多糖的工业化生产和开发利用提供了基础。
In order to improve the extraction rate of crude polysaccharide from Schizophyllum mycelium and explore the biological activity of purified intracellular polysaccharide,this study adopted the method of ultrasonic-assisted hot water extraction to extract intracellular crude polysaccharide from Schizophyllum mycelium and the extraction process was optimized by response surface method.On this basis,DEAE-52 ion-exchange column and Sephadex G-100 dextran gel column chromatography were used to separate and purify the crude polysaccharide,and then the structural characterization,antioxidant capacity and antibacterial activity of the purified polysaccharide were carried out.Results showed that the optimal extraction conditions of intracellular crude polysaccharides were as follows:Under the extraction temperature of 90℃,the water-material ratio 30:1,the ultrasonic time 30 min,the ultrasonic power 230 W.Under these conditions,the extraction rate of intracellular crude polysaccharide was 18.14%±0.33%.The purified polysaccharide fraction NSPG-1 was aβ-pyranose sugar with the molecular weight of 1.05×10^(6) Da.NSPG-1 had good antioxidant capacity and the IC_(50) values for DPPH radical,hydroxyl radical and superoxide anion radical were 6.971.07 and 11.41 mg/mL,respectively.NSPG-1 had good antibacterial activity and the IC_(50) values against Escherichia coli,Bacillus subtilis and Staphylococcus aureus were 7.56,12.54 and 10.42 mg/mL,respectively.The results would provide a basis for the industrial production and utilization of Schizophyllan.
作者
李翘楚
张璐
王红艳
王增利
丁强
王鸿磊
LI Qiaochu;ZHANG Lu;WANG Hongyan;WANG Zengli;DING Qiang;WANG Honglei(Yantai Institute of China Agricultural University,Yantai 264670,China;College of Food Science&Nutritional Engineering,China Agricultural University,Beijing 100083,China)
出处
《食品工业科技》
CAS
北大核心
2023年第4期252-260,共9页
Science and Technology of Food Industry
基金
山东省现代农业产业技术体系食用菌产业体系烟台综合试验站(SDAIT-07-14)
中国农业大学烟台研究院校级课题(YT201904)。
关键词
裂褶菌
胞内多糖
分离纯化
抗氧化性
抑菌性
Schizophyllum commune
intracellular polysaccharide
extraction and purification
antioxidant activity
antibacterial activity
