摘要
目的研究西安地区新型冠状病毒肺炎(corona virus disease 2019,COVID-19)患者康复期新型冠状病毒(2019-nCoV)核酸检测复阳情况。方法对2021年12月~2022年2月在西安秦皇医院的873例COVID-19患者康复期采集口咽和鼻咽拭子,应用实时荧光定量PCR进行2019-nCoV核酸检测,核酸复阳结果按性别、年龄、标本类型、靶标基因和Ct值与转阴时间分别进行分组比较。结果核酸检测复阳患者共201例,阳性率为23.02%。不同性别(21.58%vs 24.81%)和年龄组(24.70%vs 25.90%)核酸复阳率之间差异无统计学意义(χ^(2)=1.272,3.223,均P>0.05)。双拭子(鼻咽+口咽)联合检测复阳率明显高于单鼻咽或单口咽拭子(23.02%vs 19.01%,5.84%),且鼻咽拭子核酸复阳率明显高于口咽拭子,差异均有统计学意义(χ^(2)=8.699,121.5,69.59,均P<0.05)。双靶标基因阳性标本数明显高于单靶标N或ORF1ab基因(55.30%vs 28.57%,16.13%),且单靶标N基因阳性标本数明显高于ORF1ab基因,差异均有统计学意义(χ^(2)=31.83,72.51,9.679,均P<0.05)。不同区间Ct值组间核酸转阴时间差异有统计学意义(F=16.66,P<0.001),且转阴时间与复阳时的Ct值呈显著负相关性(r=-0.539,P<0.05)。结论COVID-19患者康复期核酸复阳率仍较高,机制和原因尚不明确,仍需加强隔离管理和健康监测。
Objective To investigate the reintroduction of 2019-nCoV nucleic acid detection in corona virus disease 2019(COVID-19)patients during convalescence in Xi’an.Methods From December 2021 to February 2022,Oropharyngeal and nasopharyngeal swabs were collected from 873 patients in Xi’an Qinhuang Hospital during the recoveryperiod.Real-time PCR was used to detect 2019-nCoV nucleic acid.The results were grouped and compared according to gender,age,specimen type,target gene,Ct value and time to turn negative.Results 201 patients were repositive,and the repositive rate was 23.02%.There was no significant difference between different genders(21.58%vs 24.81%)and age groups(24.70%vs 25.90%),and there was no significant difference between different genders and age groups(χ^(2)=1.272,3.223,all P>0.05).The combined detection rate of double swabs(nasopharynx and oropharynx)was significantly higher than that of single nasopharynx or single oropharynx swabs(23.02%vs 19.01%,5.84%),and the rate of nucleic acid reactivation of nasopharynx swabs was significantly higher than that of oropharynx swabs,and the differences were statistically significant(χ^(2)=8.699,121.5,69.59,all P<0.05).The number of double target gene positive specimens was significantly higher than that of single target N or ORF1ab genes(55.30%vs 28.57%,16.13%),and the number of single target N gene positive specimens was significantly higher than that of ORF1ab gene,and the differences were statistically significant(χ^(2)=31.83,72.51,9.679,all P<0.05).There was a significant difference in the time of nucleic acid negative transformation between different Ct values(F=16.66,P<0.001),and there was a significant negative correlation between the time of nucleic acid negative transformation and the Ct value at repositive time(r=-0.539,P<0.05).Conclusion The rate of nucleic acid reactivation was still high in COVID-19 patients during the recovery period.The mechanism and cause of reactivation are unknown,and isolation management and health monitoring are still needed
作者
赵海卫
孙珉
王娟
杨丽华
成蒙蒙
薛婧雯
王友
樊爱琳
ZHAO Hai-wei;SUN Min;WANG Juan;YANG Li-hua;CHENG Meng-meng;XUE Jin-wen;WANG You;FAN Ai-lin(Department of Clinical Laboratory Center,Xi’an Qinhuang Hospital,Xi’an 710600,China)
出处
《现代检验医学杂志》
CAS
2023年第1期175-178,共4页
Journal of Modern Laboratory Medicine
