摘要
通过梯度稀释法从连作和休耕的草莓田土壤中分离纯化得到可培养的细菌单菌落,与灰霉菌(Botrytis cinerea)或赤霉菌(Fusarium graminearum)对峙共培养,复筛得到若干株对灰霉菌或赤霉菌有拮抗作用的细菌。对拮抗效果最佳的细菌进行真菌抑菌谱的测定、植物促生效果评价及接种细菌对草莓灰霉病的影响,并对其进行分子鉴定。结果表明,试验获得1株对番茄有促生作用的细菌HPS-211,接种30 d后番茄的株高和单株鲜重平均分别提高了14.5%和26.9%,番茄叶绿素含量、叶色和植株的光泽度也有提高。对草莓苗接种HPS-211和灰霉菌B05.10发现,与阳性对照B05.10相比,接种HPS-211能降低草莓叶片灰霉病病情指数,草莓叶色、株高和光泽度与接种番茄有类似表现,处理组的草莓叶片中叶绿素和植物株高高于清水对照,达到显著性差异。HPS-211为革兰氏阴性杆菌,有鞭毛,无芽孢,经过16S rDNA分子鉴定为偶氮假单胞菌(Pseudomonas azotoformans)。
Single colonies of culturable bacteria were isolated and purified from continuous and fallow soils of strawberry fields by the gradient dilution method,co-cultured with confrontation against Botrytis cinerea or Fusarium graminearum,and several strains of bacteria antagonistic to Botrytis cinerea or Fusarium graminearum were obtained by rescreening.The bacteria with the best antagonistic effect were subjected to determination of the fungal inhibition spectrum,evaluation of plant promotion effect and its inoculated effect on Botrytis cinerea of the strawberry.The molecular identification of it was performed.The results showed that a strain of bacteria HPS-211,which had a growth-promoting effect on tomato,was obtained.The average height and fresh weight per plant of tomato were increased by 14.5%and 26.9%,respectively,30 d after inoculation with HPS-211.The chlorophyll content,leaf colour and glossiness of tomato were also increased.The inoculation of HPS-211 and B05.10 of strawberry seedlings showed that compared with the positive control B05.10,the inoculation of HPS-211 could reduce the gray mold index of strawberry leaves.The leaf color,plant height and gloss of the strawberry were similar to those of the inoculation of tomato.The chlorophyll and plant height of strawberry leaves in the treatment group were higher than those in the control,which reached a significant difference.HPS-211 was a kind of gram-negative bacterium with flagella,but without spores.Its molecular identification was Pseudomonas azotoformanss by 16S rDNA.
作者
黄薇
谢媛圆
金利容
周剑雄
熊又升
曾祥国
HUANG Wei;XIE Yuan-yuan;JIN Li-rong;ZHOU Jian-xiong;XIONG You-sheng;ZENG Xiang-guo(Institute of Plant Protection and Soil Fertilizer/Key Laboratory of Integrated Pest Management on Crops in Central Chin1,Ministry of Agriculture and Rural Affairs/Hubei Key Laboratory of Crop Disease,Insect Pests and Weeds Control,Hubei Academy of Agricultural Sciences,Wuhan 430064,China;Institute of Cash Crop Research,Hubei Academy of Agricultural Sciences,Wuhan 430064,China)
出处
《湖北农业科学》
2022年第24期78-83,共6页
Hubei Agricultural Sciences
基金
国家重点研发计划主要经济作物优质高产与产业提质增效科技创新重点专项(2020YFD100010506)
农业部华中作物有害生物综合治理重点实验室/农作物重大病虫草害防控湖北省重点实验室开放基金课题(2020ZTSJJ2)
湖北省农业科技创新中心项目(2020-620-003-001)。
