摘要
目的探讨应用小檗碱(BBR)处理对高脂饮食诱导的非酒精性脂肪性肝病(NAFLD)大鼠肝组织炎症和肠道菌群的影响.方法将45只SD大鼠随机分为对照组、模型组和小檗碱处理组,每组15只.在模型组和小檗碱处理组,采用高脂饮食喂养诱导NAFLD模型.实验结束时取肝组织行病理学检查,取血清检测空腹血糖(FPG)和空腹胰岛素(FINS),计算胰岛素抵抗指数(IRI);采集粪便,采用16SrRNA序列分析法检测肠道菌群变化;采用ELISA法检测血清白细胞介素-6(IL-6)、IL-10和肿瘤坏死因子-α(TNF-α)水平.结果在高脂喂养16 w末,取2只动物肝组织检查,提示造模成功.在实验结束时,小檗碱处理组动物肝组织学病变明显改善;小檗碱处理组大鼠血清ALT、AST和LDL水平分别为(78.0±6.0)IU/L、(119.6±8.8)IU/L和(61.3±5.0)mg/dL,显著低于模型组[分别为(211.5±9.0)IU/L、(312.6±9.7)IU/L和(97.6±8.9)mg/dL,P<0.05],而血清HDL水平为(70.0±5.4)mg/dL,显著高于模型组[(14.6±5.7)mg/dL,P<0.05];小檗碱处理组FPG、FINS和IRI分别为(4.1±0.5)mmol/L、(12.7±0.9)mU/L和(2.8±0.4),显著低于模型组[分别为(5.9±0.9)mmol/L、(19.3±1.1)mU/L和(4.6±1.0),P<0.05];小檗碱处理组大鼠肠道毛螺旋菌属和梭菌属水平分别为(5.6±0.5)和(2.0±0.4),显著低于模型组[分别为(13.4±1.3)和(7.2±0.6),P<0.05],而瘤胃球菌和乳酸菌属水平分别为(2.4±0.5)和(2.9±0.5),显著高于模型组[分别为(1.0±0.2)和(1.1±0.2),P<0.05];小檗碱处理组大鼠血清IL-6和TNF-α水平分别为(52.1±9.8)pg/mL和(70.0±17.3)pg/mL,显著低于模型组[分别为(80.3±21.6)pg/mL和(120.8±22.6)pg/mL,P<0.05],而血清IL-10水平为(6.1±2.7)pg/mL,显著高于模型组[(3.4±1.8)pg/mL,P<0.05].结论小檗碱可以通过调节肠道菌群改善NAFLD大鼠肝组织炎症反应,有效抑制细胞因子活动水平.
Objective The purpose of this experiment was to explore the effect of berberine(BBR)on liver inflammation reaction and intestinal flora changes in rats with non-alcoholic fatty liver disease(NAFLD).Methods Forty-five SD rats were randomly divided into control,model and and BBR-intervened groups,with 15 rats in each group.The NAFLD models were established by high-fat die feeding.The liver pathological changes was observed,and fasting blood glucose(FPG),fasting insulin(FINS)levels and insulin resistance index(IRI)were detected by automatic biochemical analyzer.The fecal 16SrRNA sequence were detected,and serum interleukin-6(IL-6),IL-10 and tumor necrosis factor-α(TNF-α)levels were detected by ELISA.Results At the end of 16 week experiment,the NAFLD model was successfully established as proved in two rats;at the end of the e xperiment,the liver steatosis and inflammatory reactions improved greatly in BBR-intervened rats as compared to in the model;serum ALT,AST and LDL levels in the BBR-intervened group were(78.0±6.0)IU/L,(119.6±8.8)IU/L and(61.3±5.0)mg/dL,significantly lower than[(211.5±9.0)IU/L,(312.6±9.7)IU/L and(97.6±8.9)mg/dL,respectively,P<0.05],while serum HDL level was(70.0±5.4)mg/dL,significantly higher than[(14.6±5.7)mg/dL,P<0.05]in the model;the FPG,FINS and IRI in BBR-intervened group were(4.1±0.5)mmol/L,(12.7±0.9)mU/L and(2.8±0.4),significantly lower than[(5.9±0.9)mmol/L,(19.3±1.1)mU/L and(4.6±1.0),respectively,P<0.05]in model;the fecal Lachnospira and Clostridium in BBR-intervened group were(5.6±0.5)and(2.0±0.4),significantly lower than[(13.4±1.3)and(7.2±0.6),P<0.05],while the Ruminococci and Lactic acid bacteria were(2.4±0.5 and(2.9±0.5)),significantly higher than[(1.0±0.2)and(1.1±0.2),P<0.05]in the model;serum IL-6 and TNF-αlevels in BBR-intervened group were(52.1±9.8)pg/mL and(70.0±17.3)pg/mL,both significantly lower than[(80.3±21.6)pg/mL and(120.8±22.6)pg/mL,P<0.05],while serum IL-10 level was(6.1±2.7)pg/mL,significantly higher than[(3.4±1.8)pg/mL,P<0.05]in the model.
作者
黄淡霞
招志辉
萧月兴
何艳青
Huang Danxia;Zhao Zhihui;Xiao Yuexing(Department of Pharmacy,First Affiliated Hospital,Guangzhou University of Traditional Chinese Medicine,Guangzhou 510405,Guangdong Province,China)
出处
《实用肝脏病杂志》
CAS
2023年第1期23-26,共4页
Journal of Practical Hepatology
基金
广东省中医药管理局科研项目(编号:20201096)
关键词
非酒精性脂肪性肝病
肝组织炎症
小檗碱
肠道菌群
高脂饮食
大鼠
Non-alcoholic fatty liver diseases
Liver inflammation
Berberine
Intestinal flora
High-fat diet
Rats
