木犀草素通过TLR4/NF-κB途径对哮喘模型幼鼠炎症的影响被引量：5

Effects of Luteolin on Inflammation via TLR4/NF-κB Pathway in Young Rats in an Asthma Model

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摘要目的:探究木犀草素(luteolin,LT)通过TLR4/NF-κB途径对OVA诱导幼鼠哮喘模型的炎症的影响。方法:将40只SD雌性小鼠随机分为正常组、模型组、木犀草素组。小鼠通过第1d、7d腹腔注射卵清蛋白的乳化液,第15d、16d、17d、18d、19d雾化吸入含3%OVA的生理盐水,建立哮喘模型。第15d,LT组每天腹腔注射1mg/kg、2 mg/kgLT;模型组、正常组注射等体积生理盐水。第30d采用小鼠无创肺功能仪检测小鼠气道高反应,ELISA测血清IL-2、IL-4浓度,HE染色观察肺组织炎症变化,RT-PCR检测肺组织TLR4、NF-κB的RNA表达,免疫印迹检测肺组织TLR4/NF-κBpP65蛋白表达。结果:木犀草素组的气道高反应和血清IL-4浓度低于模型组,血清IL-2浓度高于模型组。与模型组比,肺组织中,木犀草素组肺部炎症改善,木犀草素组TLR4、NF-κB的mRNA和蛋白表达低于模型组。结论:木犀草素通过调控TLR4/NF-κB途径抑制幼鼠哮喘模型的炎症反应。 Objective:To investigate the effect of luteolin(LT)on inflammation via the TLR4/NF-κB pathway in an OVA-in⁃duced asthma model in young rats.Methods:40 SD female pups were randomly divided into normal group,model group,and ligno⁃caine group.The asthma model was established in rats by intraperitoneal injection of emulsion of ovalbumin on day 1 and 7,and nebulized inhalation of saline containing 3%OVA on day 15,16,17,18 and 19.On the 15th day,1 mg/kg and 2 mg/kg lignocaine were injected in the lignocaine group,and equal volumes of saline were injected in the model and normal groups,respectively.On the 30th day,the airway hyperresponsiveness of mice was detected by non-invasive lung function instrument,serum IL-2 and IL-4 concentrations were measured by ELISA,inflammatory changes in lung tissues were observed by HE staining,RNA expression of TLR4 and NF-κB in lung tissues was detected by RT-PCR,and TLR4/NF-κB pP65 protein expression in lung tissues was detected by immunoblotting.Results:The airway hyperresponsiveness and serum IL-4 concentrations were lower in the lignocaine group than in the model group,and serum IL-2 concentrations were higher than in the model group.Compared with the model group,in lung tissue,lung inflammation was improved in the lignocaine group,and mRNA and protein expression of TLR4 and NF-κB were lower in the lignocaine group than in the model group.Conclusion:Lignocaine suppresses inflammatory responses in a young mouse asthma model by modulating the TLR4/NF-κB pathway.

作者刘克新龚本新何飞雪黎胜年郑英雷华凤 LIU Ke-xin;GONG Ben-xin;HE Fei-xue(Department of Pediatrics,East Campus of Shenzhen Hospital(Guangming),Universi-ty of Chinese Academy of Sciences,Shenzhen Guangdong,518000,China)

机构地区中国科学院大学深圳医院(光明)东院区儿科中国科学院大学深圳医院(光明)妇幼保健部

出处《黑龙江医学》 2023年第2期133-136,共4页 Heilongjiang Medical Journal

关键词木犀草素下游靶点核因子κB蛋白哮喘幼鼠炎症 Luteolin Downstream target nuclear factor-κB protein Asthma Young rat Inflammation

分类号 R562.25 [医药卫生—呼吸系统]