摘要
目的研究不同应激时间后大鼠相关脑区内神经炎症及脑区代谢的动态变化以及两者的相关性。方法将20只SD大鼠随机分成模型组(n=10)和对照组(n=10)。模型组采取孤养与慢性不可预见性轻度应激(chronic unpredictable mild stress,CUMS)相结合的方法建立应激模型,持续6周。实验前及第2、4、6周末对大鼠测定体质量及行为学指标,同时采用18氟标记的吡唑并嘧啶类的TSPO配体(^(18)F-translocator protein radioligand of pyrazolopyrimidines,^(18)F-DPA-714)及18氟标记的氟代脱氧葡萄糖(^(18)F-fluorodeoxyglucose,^(18)F-FDG)扫描检测相关脑区内神经炎症及脑区代谢的动态变化。结果在给予应激后,第4周、第6周模型组大鼠与对照组比较,体质量增加减少,不动时间延长,糖水偏好百分比减少(P<0.05)。应激2周后,海马、前额叶^(18)F-DPA-714平均标准摄取值(mean of standard uptake value,SUVmean)开始增加(P<0.05),第4周、第6周进一步增加(P<0.05)。扣带回皮质和岛叶在应激4周末^(18)F-DPA-714 SUVmean开始增加(P<0.05),第6周进一步增加(P<0.05)。同期在这些脑区中^(18)F-FDG SUVmean逐渐下降,这与相关脑区的神经炎症变化趋势具有一致性。结论抑郁大鼠中^(18)F-DPA-714及^(18)F-FDG随着应激时间延长呈一定规律性动态变化,此可能为抑郁症病理机制的特点。
Objective To investigate the dynamic changes of neuroinflammation and brain metabolism in related brain regions of rats after different stress time and their correlation.Methods Twenty SD rats were randomly divided into model group(n=10)and control group(n=10).The model group was established by the combination of bleed solitarily and chronic unpredictable mild stress(CUMS),which lasted for 6 weeks.The body weight and behavioral indexes of rats were measured before the experiment and at the 2nd,4th and 6th weekend.Meanwhile,^(18)F-DPA-714 and^(18)F-FDG scans were used to detect the dynamic changes of neuroinflammation and brain metabolism in related brain regions.Results At 4 and 6 weeks after stress,compared with the control group,the body weight of the model group was lower than the control group.The immobility time prolonged,and the percentage of sucrose preference decreased(P<0.05).At the same time,our results showed that^(18)F-DPA-714 SUVmean in hippocampus and prefrontal lobe began to increase after 2 weeks of stress and further increased at 4 and 6 weeks(P<0.05).^(18)F-DPA-714 SUVmean in cingulate gyrus and insular lobe began to increase after 4 weeks of stress and further increased at 6 weeks.During the same period,^(18)F-FDG SUVmean decreased gradually in these brain regions,which was consistent with the trend of neuroinflammation in related brain regions.Conclusion In depressive rats,^(18)F-DPA-714 and^(18)F-FDG showed certain regular dynamic changes with the extension of stress time,which may be the characteristics of the pathological mechanism of depression.
作者
陈雅洁
李月峰
CHEN Yajie;LI Yuefeng(Department of Medical Imaging,Afiliated Hospital of Jiangsu University,Jiangsu 212001,China)
出处
《医学研究杂志》
2022年第12期32-37,共6页
Journal of Medical Research
基金
国家自然科学基金资助项目(81301194)。
