摘要
随着转基因植物种植规模不断扩大,对其中转基因成分检测也提出了新的要求。近年来,数字PCR作为一种新兴的分子生物学技术在其检测方面得到了广泛应用。本文主要介绍了数字PCR的原理及数字PCR在转基因检测中的应用优势,并对转基因植物检测中的转基因成分定量检测、标准物质制备与定值及外源基因拷贝数鉴定等应用进行了总结。
In response to the growing plantation of genetically modified plants,new demands for detecting transgenic components have arisen.Recent years have witnessed a rapid increase in the use of digital PCR to detect transgenic components as a new molecular biology technology.This paper introduces the concept of digital PCR as well as its advantages in the detection of transgenic components.It also summarizes the application of digital PCR in quantitative analysis of transgenic components,preparation of reference materials,and determination of foreign gene copy numbers in genetically modified plants.
作者
游英华
赵志
李亚楠
宋治国
常洪雷
YOU Yinghua;ZHAO Zhi;LI Yanan;SONG Zhiguo;CHANG Honglei(Pro-life(Henan)Biotechnology Co.,Ltd,Zhengzhou 450008,China;Pro-life(Hebei)Biotechnology Co.,Ltd,Shijiazhuang 050221,China;Pro-life Biotechnology Co.,Ltd,Beijing 100081,China)
出处
《中国生物防治学报》
CSCD
北大核心
2022年第5期1143-1148,共6页
Chinese Journal of Biological Control
关键词
转基因植物
数字PCR
检测方法
genetically modified plants
digital PCR
detection method
