摘要
真菌所处环境的pH一直处于不断变化之中,这对真菌的生长发育具有重要的影响。真菌为了适应不断变化的环境,进化出了pH响应信号途径(即Pal途径),而PacC是该途径中的关键调节因子。针对绿色木霉TvPacC基因的功能进行分析。通过融合PCR结合原生质体转化技术构建了绿色木霉Tv-1511-△TvPacC缺失突变菌株,研究了TvPacC基因在木霉适应碱性环境和木霉菌株产碱性蛋白酶中的功能和应用。结果表明:相比较于野生型Tv-1511,Tv-1511-△TvPacC菌株具有更好的碱性环境的适应能力,而且敲除了TvPacC基因显著增强了木霉菌株产碱性蛋白酶的能力,提高了发酵液中碱性蛋白酶的产量和活性。因此,TvPacC在提高绿色木霉碱性环境的适应能力和木霉菌株产碱性蛋白酶能力等方面起着重要作用。
The pH of the environment where the fungus is located has been constantly changing,which exert important impacts on the growth and development of the fungi.In order to adapt the ever-changing environment,fungi have evolved a pH-response signal pathway(the Pal pathway),in which pacC acts as a key regulator.The function and application of TvPacC gene in Trichoderma viride Tv-1511 was analyzed,including alkaline stress resistance and alkaline protease production,by constructions of the deletion mutants(Tv-1511-△TvPacC)of TvPacC in Tv-1511 using fusion PCR technology combined with protoplast transformation method.The results show that compared with wild-type Tv-1511,Tv-1511-△TvPacC strains obtains the improved adaptability of Tv-1511 to alkaline environment.Moreover,the deletion of TvPacC resulted in the elevated production and activity of alkaline protease.In all,TvPacC gene plays important roles in the alkaline stress tolerance and alkaline protease production.
作者
张豪
李哲
郑泽慧
王庆玲
ZHANG Hao;LI Zhe;ZHENG Ze-hui;WANG Qing-ling(Biology Institute,Qilu University of Technology(Shandong Academy of Sciences),Jinan 250014,China)
出处
《科学技术与工程》
北大核心
2022年第36期15959-15966,共8页
Science Technology and Engineering
基金
山东省自然科学基金(ZR202102270296)
国家自然科学基金(21607169,3227010260)
济南市创新团队项目(2019GXRC033)
山东省科学院青年科学基金(2020QN0019)。
关键词
绿色木霉
pH响应因子
基因功能
基因编辑
抗碱特性
碱性蛋白酶
Trichoderma viride
pH response factor
gene function
gene editing
alkaline stress tolerance
alkaline protease
