摘要
目的:基于数据挖掘分析双相情感障碍(BD)与抑郁症背外侧前额叶皮质(DLPFC)转录组差异性分子表达,分析两者差异性基因相关通路及其与免疫、代谢的关系。方法:以“bipolar disorder”、“depression”、“sequencing”为关键词,在GEO数据库检索。从该基因库中分别提取6例对照和7例BD患者、29例对照和30例抑郁症患者的DLPFC转录组数据;240例健康对照和240例BD患者的外周血转录组数据,利用DESeq2软件计算差异表达基因,基于生物学过程(BP)采用GO功能分析、差异分析、交集分析的方法,分析2种疾病差异性基因相关通路及其与免疫、代谢的关系。结果:BD患者DLPFC脑区显著高表达基因总共有384个,显著低表达基因总共有117个。抑郁症患者DLPFC脑区显著高表达基因总共有165个,显著低表达基因总共有316个。BD患者和抑郁患者的脑区与正常对照相比都会发生神经系统的异常,而抑郁多富集免疫炎症相关通路,BD多富集在代谢相关通路。基因交集结果显示,抑郁患者差异基因中有74个免疫相关基因和20个代谢相关基因;而BD患者差异基因中有42个免疫相关基因和51个代谢相关基因。BD患者DLPFC脑区、外周血存在着51个与代谢相关差异性基因,外周血转录组数据验证代谢通路富集结果显示脂肪酸生物合成、泛醌等萜醌生物合成、硒化合物代谢、其他聚糖降解、其他类型的O.聚糖生物合成异常。结论:DLPFC转录组结果提示2种精神疾病在免疫、代谢相关功能的基因中发生异常比例不同,抑郁容易出现免疫相关异常,BD更容易出现代谢相关基因异常。
Objective:To analyze using data mining the differential molecular expression of transcriptome in the dorsal lateral prefrontal cortex(DLPFC)in bipolar disorder(BD)and depression,and to analyze the differential gene correlation pathways in the two disorders and their relationship with immunity and metabolism.Methods:Using"bipolar disorder","depression",and"sequencing"as keywords,the GEO(Gene Expression Omnibus)database was searched.The DLPFC transcriptome data of 6 controls and 7 BD patients and 29controls and 30 depression patients were extracted from the gene bank;the peripheral blood transcriptome data of 240 healthy controls and 240 BD patients were calculated using DESeq2 software.Based on the Biological Process(BP),GO function analysis,differential analysis,and intersection analysis were used to analyze the differential gene-related pathways of the two diseases and their relationship with immunity and metabolism.Results:There were 384 significantly overexpressed genes and 117 significantly underexpressed genes in the DLPFC brain region of BD patients.A total of 165 genes were significantly overexpressed and 316 genes were significantly underexpressed in the DLPFC brain region of depression patients.It was found that the brain regions of both BD patients and depression patients had neurological abnormalities compared with normal controls;depression was mostly enriched in immune-inflammation-related pathways,and BD was mostly enriched in metabolism-related pathways.The results of gene intersection showed that there were 74 immune-related genes and 20 metabolism-related genes in the differential genes of depression patients,while there were 42 immune-related genes and 51 metabolism-related genes in the differential genes of BD patients.There were 51 differential genes related to metabolism in the DLPFC brain region and peripheral blood of BD patients.The peripheral blood transcriptome data verified that the metabolic pathway enrichment results showed abnormalities in fatty acid biosynthesis,ubiquinone and other
作者
刘芳
杨萍
王枭冶
肖剑英
石璐
LIU Fang;YANG Ping;WANG Xiao-zhi;XIAO Jian-ying;SHI Lu(Department of Psychiatry,Hunan Brain Hospital,Changsha 410007,China)
出处
《神经损伤与功能重建》
2023年第1期28-32,共5页
Neural Injury and Functional Reconstruction
基金
湖南省卫生计生委科研课题(No.202103090662,202203094023)
湖南省临床医学研究中心(2021SK4022)
湖南省重点研发项目(2020SK2123)。
关键词
双相情感障碍
抑郁症
数据挖掘
背外侧前额叶皮质
代谢
bipolar disorder
depression
data mining
dorsal lateral prefrontal cortex
metabolism
