摘要
[目的]了解2021年10月广西某猪场引进仔猪的死亡原因。[方法]采用细菌学和分子生物学方法进行诊断,并对猪蓝耳病病毒与猪圆环病毒进行基因组层面的比较分析。[结果]本次疫病为猪蓝耳病病毒(PRRSV)、猪圆环病毒(PCV2、PCV3)型与大肠杆菌混合感染引起。对基因组比较发现,PRRSV病毒编码GP2~GP5蛋白对应的基因区域易发生突变,但编码ORF区蛋白的基因较为保守;对NCBI数据库中2022年1-5月62份PCV基因组进行分析发现,我国出现3个变种。[结论]PRRSV病毒结构更复杂,对其ORF1a、ORF1b 2个高度保守与稳定的核心区域进行PCR检测,同时相对保守的GP5糖基化包膜蛋白区域可以设计快检试剂的开发;而PCV病毒在全基因组上容易发生变异,这增加了对新型PCV变异毒株的检测难度。
[Objectives] In order to understand the causes of death of piglets introduced to a pig farm in Guangxi in October 2021. [Methods] Bacteriological and molecular biology methods were used to diagnosis, and porcine PRRS virus and porcine circovirus were compared and analysed at the genome level.[Results] The disease was caused by mixed infection of porcine PRRS virus(PRRSV), porcine circovirus(PCV2, PCV3) and Escherichia coli. The genome comparison showed that the gene region corresponding to the GP2-GP5 protein encoded by PRRSV was prone to mutation, but the gene encoding the ORF region protein was more conserved. And 62 PCV genomes from January to May 2022 in the NCBI database were analyzed and found that 3 variants appeared in China. [Conclusions] PRRSV has a more complex structure, with two highly conserved and stable core regions of ORF1a and ORF1b for PCR detection, while the relatively conserved GP5 glycosylated envelope protein region can be designed for the development of rapid detection reagents. PCV viruses were prone to mutation on the whole genome,which increases the difficulty of detecting new PCV variant strains.
作者
李剑静
LI Jianjing(Guangxi Cenxi Center for Animal Disease Prevention and Control,Cenxi 543200,China)
出处
《养殖与饲料》
2023年第2期13-20,共8页
Animals Breeding and Feed
基金
南宁市优秀青年科技创新创业人才培育项目(RC20190102)。
