摘要
目的通过生物信息学方法探索婴幼儿血管瘤(IH)的潜在生物标志物。方法2022年2月从GEO数据库中下载基因表达谱数据集(GSE127487)。利用R语言limma软件包分析IH组和健康对照组差异表达基因(DEGs)。对DEGs进行基因本体和京都基因与基因组百科全书富集分析。通过STRING分析蛋白质互作网络,利用Cytoscape软件根据不同算法筛选关键基因——hub基因。计算各个hub基因在IH组和健康对照组中的表达,最后通过受试者工作特征(ROC)曲线验证hub基因的诊断价值。结果GSE127487中有416个DEGs,主要富集于血管发育、血管生成、心血管系统的发育等,参与细胞外基质结构形成、分子功能调控、生长因子结合等,富集于白细胞跨内皮的迁移、细胞黏附分子、血管平滑肌收缩等信号通路。共筛选出PECAM1、CD34、CDH5、TEK、THY15个hub基因,并且在IH组中的表达均上调,且ROC曲线的曲线下面积均大于0.7。结论IH的hub基因与疾病发生、发展相关,是IH潜在的生物标志物,有望成为IH诊治的靶点。
Objective To explore the potential biomarkers of infantile hemangioma(IH)by bioinformatics methods.Methods The gene expression profile dataset(GSE127487)was downloaded from the GEO database in February 2022.The differentially expressed genes(DEGs)in the IH group and the healthy control group were analyzed with limma software package of R.Gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses were performed on DEGs.The protein interaction network was analyzed by STRING,and hub gene,the key gene,was selected by different algorithms using Cytoscape software.The expression of each hub gene in the IH group and the healthy control group was calculated,and finally the diagnostic value of hub gene was verified by receiver operating characteristic(ROC)curve.Results There were 416 DEGs in GSE127487,which were mainly concentrated in vascular development,angiogenesis,development of cardiovascular system,etc,involved in the formation of extracellular matrix structure,molecular function regulation,growth factor binding,etc,and were enriched in signaling pathways such as leukocyte transendothelial migration,cell adhesion molecules,and contraction of vascular smooth muscle.A total of five hub genes,including PECAM1,CD34,CDH5,TEK and THY1,were screened,and their expressions were up-regulated in the IH group,and the area under the ROC curve was greater than 0.7.Conclusion The hub gene of IH is related to the occurrence and development of disease,and is a potential biomarker of IH,which is expected to become a target for diagnosis and treatment of IH.
作者
何丹华
陈知英
陈国菊
HE Danhua;CHEN Zhiying;CHEN Guoju(Department of Cosmetic Dermatology,Shenzhen Baoan Women's and Children's Hospital,Jinan University,Shenzhen,Guangdong 518102,China)
出处
《现代医药卫生》
2023年第2期199-203,210,共6页
Journal of Modern Medicine & Health
基金
广东省深圳市宝安区医疗卫生基础研究项目(2018JD173)。
