摘要
为了明确D-氨基酸增强杀菌剂的生物膜驱散效果及杀菌机理,采用不同D-氨基酸与传统杀菌剂三丁基正十四烷基氯化膦(TTPC)、四羟甲基硫酸磷(THPS)以及抗菌肽组成复配杀菌剂,通过失重实验、电化学测试、表面分析等手段研究了复配杀菌剂对碳钢表面上的硫酸盐还原菌(SRB)、铁氧化菌(IOB)、SRB+IOB混合菌的杀菌缓蚀效果,明确了D-氨基酸驱散生物膜的行为与作用机理。结果表明:SRB和IOB发生协同作用,在碳钢试样表面形成致密的生物膜,其不仅提供了适合SRB生长的厌氧环境,还对SRB起到一定的保护作用,导致SRB+IOB混合菌造成的腐蚀最为严重,而D-氨基酸释放出的生物膜分散信号因子可改变细菌细胞壁肽聚糖成分以及调节细胞基因表达方式,通过其与细菌蛋白质结合来抑制生物膜形成,并使已有生物膜主动从碳钢表面分散脱落,破坏SRB与IOB所构成的氧浓差环境,在很大程度上抑制了因细菌所产生的胞外聚合物(EPS)导致的金属腐蚀加剧,进而使得杀菌剂能更好地杀灭生物膜下的细菌,对混合菌生物膜中SRB和IOB的杀菌率分别高达100%、82.60%,表明D-氨基酸通过驱散生物膜行为对杀菌剂起到了很好的杀菌增强效果。
In order to clarify the biofilm dispersing effect and bactericidal mechanism of D-amino acid enhanced biocide,different D-amino acids were used in this work to form a compound biocides with traditional biocides tributyl tetradecyl phosphonium chloride(TTPC),tetramethyl-phosphate sulfate(THPS)and antimicrobial peptides.By means of weightloss experiment,electrochemical test and surface analysis,the bactericidal inhibition effect of compound biocide on sulfate reducing bacteria(SRB),iron oxidizing bacteria(IOB)and the mixture of SRB+IOB on the surface of carbon steel was studied,and the behavior and mechanism of dispersive biofilm by D-amino acid were determined.The results show that SRB and IOB cooperate to form dense biofilms on the surface of carbon steel samples,which not only provides an anaerobic environment suitable for the growth of SRB,but also plays a certain protective role on SRB,which will lead to the most serious corrosion caused by the mixed bacteria of SRB+IOB.The biofilm dispersal signal factor released by D-amino acid can change the peptidoglycan composition of bacterial cell wall and regulate the expression of cell genes,inhibit the formation of biofilm by binding with bacterial proteins,and make the existing biofilm actively disperse off the surface of carbon steel,and destroy the oxygen concentration difference environment formed by SRB and IOB.D-amino acid to a large extent inhibits the aggravation of metal corrosion caused by extracellular polymeric substances(EPS)produced by bacteria,and thus enables biocides to better kill bacteria under the biofilm.The biocidal rates of SRB and IOB in the mixed biofilm are up to 100%and 82.60%,respectively.The results showed that D-amino acid had a good bactericidal enhancement effect on the biocide by dispersing the biofilm behavior.
作者
胥聪敏
高豪然
朱文胜
杨兴
陈月清
王文渊
XU Congmin;GAO Haoran;ZHU Wensheng;YANG Xing;CHEN Yueqing;WANG Wenyuan(School of Materials Science and Engineering,Xi an Shiyou University,Xi'an 710065,China;CNOOC Changzhou Paint and Coating Industry Research Institute Co.,Ltd.,Changzhou 213000,Jiangsu,China)
出处
《材料导报》
EI
CAS
CSCD
北大核心
2023年第1期239-245,共7页
Materials Reports
基金
陕西省重点研发计划项目(2020GY-234)
国家自然科学基金(51974245,21808182)
西安石油大学材料科学与工程学院,西安市高性能油气田材料重点实验室,西安石油大学“材料科学与工程”省级优势学科(YS37020203)
西安石油大学研究生创新与实践能力培养项目(YCS20112020,YCS20112021)。
