摘要
【目的】叶片受害虫取食后诱导生理防御响应是植物抵御昆虫危害的关键机制。【方法】以樟树(Cinnamomum camphora)幼苗及其食叶害虫樟叶蜂(Mesoneura rufonota)为研究体系,设置种群密度20(20头/株)、种群密度40(40头/株)和对照(无虫害)等3种处理的盆栽控制试验,于处理结束后第1天(D1)和第10天(D10)采集害虫危害的樟树叶片,研究不同种群密度樟叶蜂取食对樟树叶片其次生代谢物质(总酚、单宁)、植物激素(茉莉酸、茉莉酸甲酯)和营养成分(总糖、氨基酸)含量以及樟树幼苗生物量的影响,并探究樟树幼苗生物量与叶片中3种防御性物质之间的关联。【结果】与对照相比,在D1时种群密度20显著降低叶片总酚、茉莉酸甲酯和总糖含量,分别为19.3%、5.3%和27.3%,显著增加茉莉酸和氨基酸含量,分别为64.1%和45%;种群密度40显著降低叶片茉莉酸甲酯含量16.0%,显著增加总糖含量26.8%。D10时,种群密度20显著降低茉莉酸甲酯含量16.2%,显著增加茉莉酸和氨基酸含量,分别为127.6%和47.7%;种群密度40虫害显著降低茉莉酸甲酯含量25.1%,显著增加总酚、茉莉酸和总糖含量,分别为20.1%,18.3%和25.4%。与对照相比,种群密度20和种群密度40分别显著降低叶片生物量30.4%和46.8%,分别降低幼苗总生物量10.6%和14.2%。【结论】两种不同种群密度樟叶蜂取食后叶片的生理防御响应有别,总酚和总糖含量因受种群密度40虫害显著增加,而种群密度20虫害往往减少;2个观测时间叶片对虫害的响应也存在差异,其中单宁、总糖含量随着时间延长而增加,茉莉酸反之。昆虫取食诱导叶片次生代谢物质、植物激素和营养成分等防御性物质的变化,生理防御性物质的协同和权衡因虫害密度而不同,叶片虫害防御机制需进一步研究。
[Objective]Leaf physiologically defense is a key mechanism for plant defense in response insect pests.[Methods]The seedings of Cinnamomum Camphora and pest insect of Mesoneura Rufonota were set as research objects,which were treated with three population densities including 20 heads/plant(PD 20),40 heads/plant(PD 40)and CK(0 heads/plant).On the first day(D1)and the tenth day(D10)after the end of experimental treatment,the leaves of the Cinnamomum Camphora infested by Mesoneura Rufonota were collected.We investigated the secondary metabolites(total phenols,tannins),phytohormones(jasmonic acid,methyl jasmonate),nutrients(total sugars,amino acids)of Cinnamomum camphora leaves and seedling biomass of Cinnamomum camphora and explored the relationship between the biomass of Cinnamomum camphora seedling and the three defensive substances in leaves.[Results]At the D1,compared with PD0 treatment,PD20 treatment significantly decreased leaf total phenol,methyl jasmonic and total sugar contents by 19.3%,5.3%and 27.3%,respectively.PD20 treatment significantly increased jasmonic acid and amino acid contents by 64.1%and 45%,respectively.Meanwhile PD40 treatment significantly decreased methyl jasmonate contens in leaves by 16.0%and significantly increased total sugar contents by 26.8%.Compared with CK,PD20 treatment significantly decreased the content of methyl jasmonate by 16.2%,and significantly increased the content of jasmonic acid and amino acids by 127.6%and 47.7%,respectively.At the D10,PD 40 treatment significantly decreased the content of methyl jasmonate by 25.1%and significantly increased total phenol,jasmonic acid and total sugar contents by 20.1%,18.3%and 25.4%,respectively.Compared with CK,PD20 and PD 40 significantly reduced leaf biomass by 30.4%and 46.8%,and reduced total seedling biomass by 10.6%and 14.2%,respectively.Correlation analysis showed that at D1,jasmonic acid was significantly positively correlated with amino acids,and both total phenols and tannins were significantly negatively correlated with amino
作者
程柯方
刘俏
程远东
梁宽
陈伏生
CHENG Kefang;LIU Qiao;CHENG Yuandong;LIANG Kuan;CHEN Fusheng(Jiangxi Provincial Key Laboratory of Silviculture,College of Forestry,Jiangxi Agricultural University,Nanchang 330045,China;Key Laboratory of National Forestry and Grassland Administration on Forest Ecosystem Protection and Restoration of Poyang Lake Watershed,Jiangxi Agricultural University,Nanchang 330045,China)
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2022年第6期1457-1465,共9页
Acta Agriculturae Universitatis Jiangxiensis
基金
国家自然科学基金项目(32171759)。
关键词
樟叶蜂
防御机制
樟树
次生代谢物质
植物激素
Mesoneura rufonota
defensive mechanisms
Cinnamomum camphora
secondary metabolites
plant hormones
