摘要
建立了一种采用紫外-可见分光光度法测定铁皮石斛多糖含量的方法。采用苯酚-硫酸法测定铁皮石斛多糖含量,考察了醇沉条件和煮沸时间对铁皮石斛多糖质量浓度的影响。结果表明,最佳醇沉和测定条件:醇沉浓度为体积分数80%乙醇,醇沉时间为16 h,煮沸时间为10 min。葡萄糖的质量在10.85~108.5μg范围内,线性关系良好,相关系数(r)为0.9999。精密度、稳定性、重复性的RSD均小于2.0%,低、高浓度的平均加标回收率分别为98.3%和97.1%,RSD分别为0.86%和0.85%。该方法快速、简单、准确、可靠,可用于测定铁皮石斛多糖的含量,可以作为评价铁皮石斛药材的产品质量标准和为化妆品美妆功能研究提供科学依据。
A ultraviolet-visible spectrophotometry method was established to determine the polysaccharide content in dendrobium officinale.The content of dendrobium officinale polysaccharide was determined by phenol-sulfuric acid method.The effects of alcohol precipitation conditions and boiling time on dendrobium officinale polysaccharide mass concentration were investigated.The results showed that the optimal alcohol settling and determination conditions were as follows:the alcohol settling concentration was 80%ethanol by volume fraction,the alcohol settling time was 16 h,and the boiling time was 10 min.Under this condition,there were good linear relationships between the mass and the absorbance value for glucose in the range of 10.85~108.5μg,the correlation coefficient(r)was 0.9999.The relative standard deviation of precision,stability and repeatability were all less than 2.0%,the average recoveries of low and high concentrations were 98.3%and 97.1%while the relative standard deviation were 0.86%and 0.85%,respectively.This method is rapid,simple,accurate and reliable for the determination of dendrobium officinale polysaccharide,which can be used as product quality standard to evaluate dendrobium officinale and provide scientific basis for the study of cosmetic function.
作者
冉金凤
于海英
李启艳
张良雨
高樱
张金岭
李俊婕
Ran Jinfeng;Yu Haiying;Li Qiyan;Zhang Liangyu;Gao Ying;Zhang Jinling;Li Junjie(Shandong Institute for Food and Drug Control,NMPA Key Laboratory for Quality Control of Cosmetics Raw Material,Jinan 250101,China;Shandong Chunxu Chemical Engineering Design Co.,Ltd.,Jinan 250014,China)
出处
《山东化工》
CAS
2022年第23期150-153,共4页
Shandong Chemical Industry
关键词
铁皮石斛多糖
紫外-可见分光光度法
醇沉条件
含量测定
dendrobium officinale polysaccharide
ultraviolet-visible spectrophotometry
alcohol precipitation condition
content determination
