摘要
OBJECTIVE: To investigate whether electroacupuncture(EA) at bilateral Tianshu(ST25) and Zusanli(ST36) acupoints could alleviate stress-induced irritable bowel syndrome(IBS) and evaluate its effect on gut microbiota and corticotropin-releasing factor(CRF). METHODS: Thirty C57BL/6 mice were randomly divided into the normal, water avoidance stress(WAS), and WAS+EA groups(10 mice per group). An experimental model of IBS was established by exposing the animals to WAS. The mice were treated with EA at the bilateral Tianshu(ST25) and Zusanli(ST36) acupoints. The abdominal withdrawal reflex test was conducted to evaluate visceral sensitivity in IBS. Gut microbiota was analyzed using 16S r RNA sequencing and analysis. The expression of CRF was determined using immuneofluorescence and quantitative real-time polymerase chain reaction. RESULTS: EA alleviated visceral hypersensitivity in a mouse model of WAS-induced IBS. It modulated the dysbiosis of gut microbiota induced by WAS. Moreover, it suppressed the WAS-induced overexpression of CRF in colon tissues. CONCLUSION: The findings of this study suggest that EA alleviated WAS-induced IBS via mechanisms possibly involving the modulation of the dysbiosis of gut microbiota and suppression of CRF expression.
基金
Supported by Natural Science Foundation-funded Project:Mechanism of Electroacupuncture Regulating CRF-NLRP6 InflammasomeRelated Pathways in Intestinal Flora Immune Dialogue (No. 81804193)
Leading Talents of Jiangsu Provincial Administration of TCM-funded Project:Clinical Effect and Mechanism of Acupuncture and Moxibustion on Irritable Bowel Syndrome (No. SLJ0206)
Peak Talent of Jiangsu Province Hospital of Chinese Medicine-funded Project:Clinical Effect and Mechanism of Acupuncture and Moxibustion in the Treatment of Functional Gastrointestinal Diseases (No. y2018rc05)
Research and Innovation Project for Postgraduates of Jiangsu Province-funded Project:Mechanism of Electroacupuncture Regulating Bile Acid Receptor to Alleviate Visceral Hypersensitivity in Irritable Bowel Syndrome (No. YCX20_1469)。
