摘要
2021年11月在沈阳市辽中区蒲河湿地公园(41°30′55″N,122°78′30″E,海拔30 m)采集到2号无尾两栖类标本,经形态特征比较确认为无尾目(Anura)蛙科(Ranidae)蛙属(Rana)物种,基于线粒体16S rRNA基因对蛙属19个物种的亲缘关系及系统发育进行分析,其与韩国及中国山东文登昆嵛山分布的韩国林蛙(R.coreana)遗传距离最近,并在最大似然树中聚为一支,应属种内关系。综合形态分析和系统发育比较,确定采集到的标本为韩国林蛙,系辽宁省两栖动物分布新记录种。本次发现将辽宁省分布的蛙属物种增至5种。
In November,2021,2 amphibians were collected from Puhe Wetland Park,Liaozhong District,Shenyang(41°30′55″N,122°78′30″E,elevation 30 m).After comparison of morphological characteristics,they were confirmed as species of Rana,Ranidae and Anura.In order to determine the taxonomic relationship of this species and further determine their distribution characteristics,we conducted in-depth research and analysis on them.We used second high-throughput sequencing to sequence the mitochondrial 16S rRNA gene,and constructed a phylogenetic tree to analyze the phylogeny of 19 species of Rana(Table 1).This species of Rana has the closest genetic distance to R.coreana from Korea and Kunyu Mountain in Shandong Province,China,and clustered into a branch in maximum likelihood tree,which suggested an intraspecies relationship(Fig.2 and Table 2).Based on morphological analysis and phylogenetic comparison,it was determined that the collected specimen was R.coreana(Fig.1).R.coreana is a new record of amphibians in Liaoning Province.The discovery brings the number of frog species in Liaoning province to 5.
作者
周圣博
于梦竹
苗青
翟强
徐树军
关萍
ZHOU Sheng-Bo;YU Meng-Zhu;MIAO Qing;ZHAI Qiang;XU Shu-Jun;GUAN Ping(College of Bioscience and Technology,Shenyang Agricultural University,Key Lab of Global Changes and Biological Invasions,Liaoning Province,Shenyang 110866;Liaoning Panjin Wetland Ecosystem National Observation and Research Station,Panjin 124112;Liaoning Shuangtai Estuary Wetland Ecosystem Research Station,Panjin 124112;Wafangdian Agricultural Technology Extension Center,Dalian 116300;State-owned Asset Management Office of Shenyang Agricultural University,Shenyang 110866,China)
出处
《动物学杂志》
CAS
CSCD
北大核心
2022年第6期944-950,共7页
Chinese Journal of Zoology
基金
2021年辽宁省普通本科高等学校校际合作项目-资源共享(2021-57)
“辽宁盘锦湿地生态系统野外科学观测研究站”建设项目。
