摘要
目的探究丁苯酞对低氧暴露大鼠所致高原脑水肿的防治作用。方法60只SD大鼠随机分为空白对照组、低氧模型组、银杏叶提取物片组(80 mg·kg-1),丁苯酞低、中、高剂量组(40、80、120 mg·kg-1),每组10只。各组灌胃4 d后置于模拟海拔6000 m人工低氧实验舱72 h建立急性高原脑水肿模型,持续给药3 d,其中空白对照组和低氧模型组给予等量0.9%氯化钠溶液灌胃。出舱后取材,以脑组织干湿重比考察脑组织含水量;采用酶联免疫吸附法测定脑组织中超氧化物歧化酶、丙二醛、谷胱甘肽过氧化物酶、过氧化氢酶等水平变化;苏木精-伊红染色观察脑组织病理形态变化。结果与空白对照组比较,低氧模型组大鼠脑含水量和组织中丙二醛显著升高(P<0.01),超氧化物歧化酶和谷胱甘肽过氧化物酶显著降低(P<0.05),而过氧化氢酶水平无显著改变;苏木精-伊红染色显示低氧模型组海马区部分细胞出现空泡,椎体层细胞水肿明显,血管周间隙明显增大。与低氧模型组比较,银杏叶提取物片组脑含水量显著降低(P<0.05),丙二醛水平显著下降(P<0.01),超氧化物歧化酶显著升高(P<0.01),谷胱甘肽过氧化物酶显著升高(P<0.05)。丁苯酞高剂量组大鼠脑含水量显著降低(P<0.01)、脑组织中超氧化物歧化酶和谷胱甘肽过氧化物酶水平均有显著升高(P<0.01)、丙二醛水平显著降低(P<0.01),丁苯酞中、低剂量组无显著改变,苏木精-伊红染色显示银杏叶提取物片组和丁苯酞中、高剂量组脑细胞肿胀数量有所减少,血管周间隙也明显改善。结论丁苯酞对低氧暴露大鼠高原脑水肿有一定的改善作用。
Objective To explore the role of butylphthalide in preventing high altitude cerebral edema induced by hypoxia exposure.Methods Sixty SD rats were randomly divided into the blank control group,hypoxia model group,Ginkgo biloba extract tablet group(80 mg·kg-1),and butylphthalein low,medium and high dose groups(40,80 and 120 mg·kg-1),with 10 rats in each.After four days of administration by gavage,each group was placed in an artificial hypoxic chamber at an altitude of 6000 m for 72 h to establish a model of acute plateau brain edema before being administered for three consecutive days.The blank control group and the hypoxic model group were given equal amounts of 0.9%sodium chloride solution by gavage.After the rats left the chamber,the water content of brain tissues was determined using the dry to wet weight ratio of brain tissues.The changes of levels of superoxide dismutase,malondialdehyde,glutathione peroxidase and catalase in brain tissues were measured by enzyme-linked immunosorbent assay while the morphological changes of brain tissues were observed via hematoxylin-eosin staining.Results Compared with the blank control group,the brain water content and level of malondialdehyde in the hypoxia model group were significantly higher(P<0.01),while levels of superoxide dismutase and glutathione peroxidase were significantly lower(P<0.05),but the level of catalase was not significantly changed.The perivascular gap was enlarged.Compared with the hypoxic model group,the brain water content of Ginkgo biloba extract tablet group was significantly lower(P<0.05),so was the level of malondialdehyde(P<0.01),but the level of superoxide dismutase was significantly higher(P<0.01)so was the level of glutathione peroxidase(P<0.05),but the brain water content of the butyl phthalide high dose group was significantly lower(P<0.01),while the levels of superoxide dismutase and glutathione peroxidase in brain tissue were significantly higher(P<0.01).The expressions of superoxide dismutase and glutathione peroxidase in brain tissue
作者
马博华
李萌
王江涛
李倩
王瑞
尹东锋
MA Bo-hua;LI Meng;WANG Jiang-tao;LI Qian;WANG Rui;YIN Dong-feng(School of Pharmacy,Shihezi University,Shihezi 832000,China;Xinjiang Military Region General Hospital of PLA,Urumqi 830000,China)
出处
《解放军药学学报》
CAS
2022年第6期501-504,共4页
Pharmaceutical Journal of Chinese People's Liberation Army
基金
军队后勤科研计划面上项目,No.CLJ20J030。
关键词
丁苯酞
高原
脑水肿
氧化应激
大鼠
butylphthalide
high altitude
brain edema
oxidative stress
rats
