摘要
背景:目前常用的膝骨关节炎动物模型制备方法主要有手术创伤和关节腔内注射蛋白酶诱导两大类。关节腔内注射药物诱导模型并不稳定,传统的手术造模方法对膝关节损伤较大,且成模过程与临床膝骨关节炎的发展过程差异较大。目的:通过不同手术方式损伤膝关节制备兔膝骨关节炎模型,探讨膝骨关节炎动物模型的最佳制备方法。方法:将16只雄性五六月龄新西兰大白兔随机分为假手术组、模型A组、模型B组、模型C组,每组4只。假手术组切开右侧膝关节内侧后立即缝合;模型A组磨损右侧膝关节半月板、磨损前交叉韧带;模型B组摘除右侧膝关节半月板、磨损前交叉韧带;模型C组摘除右侧膝关节半月板、切断前交叉韧带。术后连续3 d注射抗生素,记录一般情况。4周后通过X射线片观察关节情况,苏木精-伊红染色观察软骨组织变化,酶联免疫吸附法检测右侧膝滑膜液和血清中炎症因子水平。结果与结论:(1)模型组右侧膝关节明显出现红肿热痛情况,皮温明显高于假手术组;(2)术后模型组右侧膝关节皮温较假手术组明显升高,差异有显著性意义(P<0.05);(3)X射线片显示假手术组膝关节未见异常,模型组出现不同程度的关节面破坏及关节间隙狭窄,其中模型C组最为严重;(4)苏木精-伊红染色显示假手术组为软骨组织正常形态;模型组出现软骨细胞数量减少,层次紊乱,其中模型C组软骨结构损坏严重,可见血管生成;(5)酶联免疫吸附实验显示,模型组兔血清炎症因子水平较假手术组明显升高,差异有显著性意义(P<0.001);模型组兔右侧膝关节滑膜液炎症因子水平较假手术组明显升高,差异有显著性意义(P<0.001);(6)提示通过对各组兔膝关节一般情况、影像学及相关因子改变分析发现,以上3种手术损伤膝关节方法均能成功构建兔膝骨关节炎模型,模型C组已达到膝骨关节炎中晚期改变,模�
BACKGROUND:The two methods commonly used for preparing animal models of knee osteoarthritis are surgical trauma and intra-articular injection of protease.However,the models induced by intra-articular injection of drugs are not stable,the traditional surgical modeling method has great damage to the knee joint,and the modeling process differs greatly from the development of clinical knee osteoarthritis.OBJECTIVE:To evaluate the best preparation method for the animal model of knee osteoarthritis through establishing a rabbit knee osteoarthritis model by injuring the knee joint in different surgical ways.METHODS:Sixteen male New Zealand white rabbits aged 5-6 months were randomly divided into sham operation group,model group A,model group B and model group C,with four rats in each group.In the sham operation group,the medial side of the right knee joint was incised and immediately sutured.In the model group A,the meniscus and anterior cruciate ligament of the right knee were worn.In the model group B,the meniscus of the right knee joint was removed and the anterior cruciate ligament of the right knee was worn.In the model group C,the right knee meniscus was removed and the anterior cruciate ligament of the right knee joint was transected.Antibiotics were injected for 3 consecutive days after operation,and the general conditions were recorded.After 4 weeks,the knee joints were observed by X-ray,the changes of cartilage tissue were observed by hematoxylin-eosin staining,and the levels of inflammatory factors in the synovial fluid and serum of the right knee were detected by enzyme-linked immunosorbent assay.RESULTS AND CONCLUSION:Redness,swelling and heat pain were evident in the right knee joint of the model group,and the skin temperature in the model groups was significantly higher than that in the sham operation group.Postoperative skin temperature of the right knee joint in the model groups was significantly higher than that in the sham operation group(P<0.05).The X-ray observation showed no abnormalities in the kn
作者
张传成
沈美花
陈利锋
王华松
项阳
谭张奎
Zhang Chuancheng;Shen Meihua;Chen Lifeng;Wang Huasong;Xiang Yang;Tan Zhangkui(Wuhan University of Science and Technology,Wuhan 430065,Hubei Province,China;Central Theater Command General Hospital of the Chinese PLA,Wuhan 430070,Hubei Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2023年第23期3646-3652,共7页
Chinese Journal of Tissue Engineering Research
基金
湖北省医学青年拔尖人才(第一层次)(1371),项目负责人:陈利锋。
关键词
膝骨关节炎
动物模型
炎症
关节积液
软骨
knee osteoarthritis
animal model
inflammation
joint effusion
cartilage
