摘要
目的考察新绿原酸(5-caffeoylquinic acid,5-CQA)对血管生成的影响。方法应用人脐内皮细胞(HUVEC)、大鼠动脉环模型和转基因血管荧光斑马鱼模型考察5-CQA对血管生成的抑制作用。同时建立肿瘤细胞斑马鱼移植瘤模型,考察5-CQA对肠下静脉(subintestinal vein,SIV)生成的作用。结果5-CQA在320μmol·L^(-1)及以下浓度对HUVEC的增殖无明显影响,5-CQA能够抑制HUVEC体外迁移及细胞中VEGF、bFGF mRNA的表达;与对照组比较,56和112μmol·L^(-1)的5-CQA具有显著抑制大鼠动脉环微血管生成的作用(P<0.05);在斑马鱼血管荧光模型中56和112μmol·L^(-1)的5-CQA具有显著抑制24 h斑马鱼节间血管(intersegmental vessel,ISV)生成的作用;同时5-CQA对斑马鱼移植瘤的肠下静脉生成具有明显抑制作用(P<0.05)。结论5-CQA具有抑制血管生成的作用,其作用机制与抑制VEGFR2受体的表达有关。
Objective To investigate the effect of 5-caffeoylquinic acid(5-CQA)on angiogenesis.Methods Human umbilical endothelial cells(HUVEC),rat arterial ring model and transgenic vascular fluorescent zebrafish model were used to investigate the inhibitory effect of 5-CQA on angiogenesis;At the same time,the zebrafish transplantation tumor model was established to investigate the effect of 5-CQA on the generation of subintestinal vein(SIV).Results Compared with control group,the proliferation inhibition rate of HUVEC was not changed obviously under 320μmol·L^(-1)5-CQA.5-CQA can inhibit HUVEC migration in vitro and the expression of VEGF and bFGF mRNA in cells.Rat aortic rings assay showed the 56 and 112μmol·L^(-1)5-CQA displayed a good inhibition rate(P<0.05).The intersegmental vessels(ISV)of zebrafish were inhibited by 56 and 112μmol·L^(-1)5-CQA.Same result was obtained in xenograft model.5-CQA significantly decreased the length SIV of xenograft zebrafish embryos(P<0.05).Conclusion 5-CQA can inhibit angiogenesis via blocking VEGFR2.
作者
袁红芹
王荣春
段秀英
王梦婷
陈锡强
YUAN Hongqin;WANG Rongchun;DUAN Xiuying;WANG Mengting;CHEN Xiqiang(Shaanxi Buchang Pharmaceutical Limited Company,Xianyang 712000,China;Biology Institute,Qilu University of Technology,Jinan 250103,China;Shaanxi Institute of International Trade&Commerce,Xi'an 710075,China;Shandong Provincial Engineering Laboratory for Biological Testing Technology,Jinan 250103,China)
出处
《西北药学杂志》
CAS
2023年第1期91-95,共5页
Northwest Pharmaceutical Journal
基金
山东省重大科技创新工程项目(编号:2019JZZY020905)
国家重点研发计划项目(编号:2018YFC1707300)
齐鲁工业大学(山东省科学院)科教产融合创新试点工程项目(编号:2020KJC-ZD08)。
关键词
新绿原酸
血管生成
血管内皮生长因子
移植瘤模型
5-caffeoylquinic acid
angiogenesis
endothelial growth factor receptor
xenograft
