摘要
试验旨在研究不同青贮饲料添加剂组合对饲料桑青贮营养价值和发酵品质的影响。试验采用3×3随机区组试验设计,对照组仅添加20 g/kg葡萄糖,试验组饲料桑除添加20 g/kg葡萄糖外,分别添加由植物乳杆菌、戊糖片球菌、地衣芽孢杆菌组成的复合菌和由里氏木霉木聚糖酶、酸性纤维素酶、α-半乳糖苷酶组成的复合酶,分别添加高、中、低共3个浓度,共10个处理组。裹包青贮60 d,进行营养价值评定。结果显示:1组饲料桑的pH值、气味、色泽、综合分数均高于其他各组,评定等级为优等。各试验组饲料桑青贮的干物质(DM)含量均显著低于对照组(P<0.05)。与对照组相比,1组、2组、8组饲料桑的粗脂肪(EE)含量显著提高(P<0.05);各试验组饲料桑的中性洗涤纤维(NDF)和酸性洗涤纤维(ADF)均显著降低(P<0.05),其中1组饲料桑的NDF含量最低,4组饲料桑的ADF含量最低。与对照组相比,各试验组饲料桑的pH值均显著降低(P<0.05),且1组饲料桑的pH值最低(3.85)。与对照组相比,1组、2组、4组、5组、7组、8组饲料桑的乳酸和乙酸含量显著增加(P<0.05),氨态氮(NH_(3)-N)含量显著降低(P<0.05),且1组饲料桑的乳酸和乙酸含量最高,NH_(3)-N含量最低。研究表明,饲料桑青贮最优组合为1组,即同时添加α-半乳糖苷酶40 U/kg、酸性纤维素酶20 U/kg、里氏木霉木聚糖酶40 U/kg、植物乳杆菌5×10^(5)CFU/kg、戊糖片球菌3×10^(6)CFU/kg、地衣芽孢杆菌1×10^(8)CFU/kg以及20 g/kg葡萄糖,能够明显改善饲料桑青贮饲料品质。
The aim of the experiment was to study the effect of different silage additive combinations on nutritional value and fermentation quality of forage mulberry silage.The experiment was a 3×3 randomized block design.Control group was only supplemented with 20 g/kg glucose,while the experimental group was supplemented with 20 g/kg glucosem,also necessary to add high,medium and low concentrations of Lactobacillus plantarum,Pediococcus pentosus,Bacillus licheniformis and Trichoderma reesei xylanase,acid cellulase,α-galactosidase to treat feed mulberry,namely ten treatment groups.The nutritional value was evaluated after 60 d of package silage.The results showed that the pH value,odor,color and comprehensive score of group 1 were higher than those of other groups,and the rating grade was excellent.The DM content of experimental groups was significantly lower than that of control group (P<0.05).Compared with control group,the EE content in group 1,group 2 and group 8 was significantly increased (P<0.05),the content of NDFand ADF in experimental groups were significantly decreased (P<0.05),and the content of NDF in group 1 was the lowest,and the content of ADF in group 4 was the lowest.Compared with control group,the pH value of all experimental groups was significantly decreased(P<0.05),and the pH value of group 1 was the lowest(3.85).Compared with control group,the content of lactic acid and acetic acid in group 1,group 2,group 4,group 5,group 7 and group 8 were significantly increased (P<0.05),and the content of NH-N was significantly decreased (P<0.05),and the content of lactic acid and acetic acid in group 1 were the highest,and the content of NH-N was the lowest.The study indicates that the optimal combination of silage mulberry is group 1,α-galactosidase40 U/kg,acid cellulase 20 U/kg,Trichoderma reesei xylanase 40 U/kg,Lactobacillus plantarum 5×10^(5)CFU/kg,Pediococcus pentosus 3×10^(6)CFU/kg,Bacillus licheniformis 1×10^(8)CFU/kg and 20 g/kg glucose are added simultaneously.It can significantly improve the qu
作者
郝丽红
王彦平
王勇
王怀中
呼红梅
王诚
HAO Li-hong;WANG Yan-ping;WANG Yong;WANG Huai-zhong;HU Hong-mei;WANG Cheng
出处
《饲料研究》
CAS
北大核心
2022年第20期83-87,共5页
Feed Research
基金
山东省农业重大应用技术创新项目(项目编号:SD2019XM010)
山东省农业科技资金“园区产业提升工程(项目编号:2019YQ027)”
山东省现代农业产业技术体系牧草体系(项目编号:SDAIT-23-07)。
关键词
桑
复合菌
复合酶
营养价值
发酵品质
mulberry
compound bacterium
composite enzyme
nutritional value
fermentation quality