摘要
利用中红外光谱技术结合化学计量方法对不同产地半夏快速鉴别。采集5个不同产地共50份半夏样本的红外光谱,对原始光谱进行预处理之后,取平均光谱。比较全波段光谱之间的相似性以及二阶光谱之间的差异性,并结合主成分分析法以及聚类分析等化学计量法,探讨鉴别不同产地半夏的方法。结果显示5个产地平均光谱统计结果显示平均红外光谱相似度较高,五种产地半夏的红外光谱图进行求导,分别得到各产地的二阶红外光谱图,分析其差异性,主要集中在1690~1650 cm^(-1)、1645 cm^(-1)、1580~1510 cm^(-1)、1370~1000 cm^(-1)、820~780 cm^(-1)波段内峰强、峰形、峰数等的差异。结合主成分分析法,五种产地半夏样本在主成分空间中分布为5种不同的类别;聚类分析方法结果可将5种产地半夏划分为2个表征群,其中河南南阳与河南洛阳半夏样品、四川资阳与山西运城半夏样品首先聚为一类,而湖北天门的半夏样品处于第二表征群,说明该产地的半夏样品相似度匹配相对较远,将半夏药材ATR红外光谱与鸟苷、腺苷、琥珀酸3种标准品的ATR红外光谱进行对比分析。实现不同产地半夏的鉴别。
To identify Banxia(Pinelliae Rhizoma)from different areas by mid infrared spectroscopy and stoichiometry.The infrared spectra of 50 Banxia(Pinelliae Rhizoma)samples from 5 different areas were collected by infrared spectroscopy.After pretreatment of the original spectra,the average spectra was obtained.The similarity of the whole band spectra and the difference of the second order spectra were compared,and the methods of identifying Banxia(Pinelliae Rhizoma)from different areas were discussed by combining the principal component analysis and cluster analysis.The average spectral statistics results of five producing areas showed that the average infrared spectrum similarity was high.The infrared spectra of Banxia(Pinelliae Rhizoma)from five producing areas were derived to obtain the second-order infrared spectra of each producing area,and their differences were analyzed.The differences of peak strength,peak shape and peak number in bands 1690~1650 cm-1,1645 cm^(-1),1580~1510 cm^(-1),1370~1000 cm^(-1) and 820~780 cm^(-1) were mainly concentrated.In combination with principal component analysis,Banxia(Pinelliae Rhizoma)samples from five habitats were divided into five different categories in principal component space.The results of cluster analysis showed that Banxia(Pinelliae Rhizoma)from five areas could be divided into two representative groups,Banxia(Pinelliae Rhizoma)samples from Nanyang of Henan Province and Luoyang and Ziyang of Sichuan Province and Yuncheng of Shanxi Province were clustered in the first group,while Banxia(Pinelliae Rhizoma)samples from Tianmen of Hubei Province were in the second characterization group,indicated that the similarity of Banxia(Pinelliae Rhizoma)samples from this area is relatively far.The ATR infrared spectra of Banxia(Pinelliae Rhizoma)are compared with those of guanosine,adenosine and succinic acid to identify Banxia(Pinelliae Rhizoma)from different areas.
作者
乔璐
张雨晴
赵博宇
周春亚
刘瑞娜
韩盼盼
张瑞
董诚明
QIAO Lu;ZHANG Yuqing;ZHAO Boyu;ZHOU Chunya;LIU Ruina;HAN Panpan;ZHANG Rui;DONG Chengming(School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,Henan,China;Peking Union Medical College,Chinese Academy of Medical Sciences,Beijing 100193,China;Jiangxi University of Chinese Medicine,Nanchang 330004,Jiangsi,China;Guangzhou University of Chinese Medicine,Guangzhou 510006,Guangdong,China)
出处
《中华中医药学刊》
CAS
北大核心
2022年第11期73-76,I0026,I0027,I0028,I0029,共8页
Chinese Archives of Traditional Chinese Medicine
基金
国家重点研发计划(2017YFC1702800)
河南省重大科技专项(171100310500)
河南中医药大学博士科研基金项目(BSJJ2014-01)。
关键词
半夏
不同产地
中红外光谱
相关系数
主成分分析
聚类分析
Banxia(Pinelliae Rhizoma)
different areas
mid-infrared spectrum
coefficient of association correlation index
principal component analysis
cluster analysis
