摘要
目的:探讨尪痹胶囊对Ⅱ型胶原致大鼠滑膜炎的治疗作用。方法:雌性Wistar大鼠48只,随机分为正常组、模型组、双氯酚酸钠组(6.75 mg·kg^(-1)·d^(-1),灌胃)和尪痹胶囊低、中、高剂量组(247.5、742.5、2227.5 mg·kg^(-1)·d^(-1),灌胃)。除正常组外,其余各组大鼠均采用Ⅱ型胶原诱导大鼠滑膜炎模型,造模成功后双氯酚酸钠组和尪痹胶囊各剂量组大鼠连续给药3周,正常组和模型组给予等量羧甲基纤维素钠溶液。采用容积测量法和计分法检测各组大鼠足跖肿胀度和关节炎指数;酶联免疫吸附测定检测大鼠血清TNF-α、IL-1β、IL-10水平;苏木素-伊红染色检测滑膜组织病理学改变。结果:与模型组比较,双氯芬酸钠组和尪痹胶囊低、中、高剂量组大鼠足跖肿胀度和关节炎指数评分均有不同程度降低(P<0.05,P<0.01,P<0.001);血清TNF-α、IL-1β水平显著降低(P<0.01,P<0.001),IL-10水平显著升高(P<0.001);炎症得到不同程度缓解。结论:尪痹胶囊可改善胶原致大鼠滑膜炎症状,具有一定的治疗效果。
Objective:To investigate the therapeutic effect of Wang-Bi Capsule on collagenⅡinduced synovitis in rats.Methods:48female Wistar rats were randomly divided into control group,model group,diclofenac sodium group(6.75 mg·kg^(-1)·d^(-1),i.g.),low dose(247.5 mg·kg^(-1)·d^(-1),i.g.),medium-dose(742.5 mg·kg^(-1)·d^(-1),i.g.)and high dose(2227.5 mg·kg^(-1)·d^(-1),i.g.)of Wang-Bi Capsule groups.Except for control group,rats in the other groups were given collagenⅡto induce synovitis model.After the successful establishment of synovitis model,rats in diclofenac sodium group and each dose group of Wang-Bi capsule were treated with continous administration for 3weeks,while rats in control and model group were given equal amount of sodium carboxymethyl cellulose solution.Metatarsal swelling degree and arthritis index of rats in each group were detected by volume measurement method and scoring method.The levels of serum TNF-α,IL-1β,IL-10 in rats were measured by enzyme-linked immunosorbent assay.And the histopathological changes of synovial membrane were detected by hematoxylin-eosin staining.Results:Compared with the model group,metatarsal swelling and arthritis index scores of rats in diclofenac sodium group and low,medium and high dose of Wang-Bi Capsule groups were decreased to varying degrees,the levels of serum TNF-α,IL-1βwere decreased significantly while the level of IL-10 was increased significantly,and inflammation was relieved to varying degrees(P<0.05,P<0.01,P<0.001).Conclusion:Wang-Bi Capsule can alleviate synovial inflammation caused by collagenⅡin rats,showing its certain therapeutic effect.
作者
王妮
吴闪闪
WANG Ni;WU Shanshan(Traditional Chinese Medicine Gynecology Department,Huantai Matern&Child Health Hospital,Zibo 256400,Shandong,China;State Key Laboratory of Natural Medicines of China Pharmaceutical University,Nanjing 210009,Jiangsu,China)
出处
《上海中医药大学学报》
CAS
2022年第6期76-80,共5页
Academic Journal of Shanghai University of Traditional Chinese Medicine
基金
国家重点研发计划项目(2019YFC1711000)。
