摘要
采用3%(质量分数)葡聚糖硫酸钠(dextran sulfate sodium,DSS)诱导溃疡性结肠炎(ulcerative colitis,UC)小鼠模型,探究刺梨总多糖(Rosa roxburghii Tratt total polysaccharide,RP)和总黄酮(Rosa roxburghii Tratt total flavonoids,RF)对UC小鼠的干预效果及机制。将40只雄性小鼠(C57BL/6J)随机分成空白组、模型组、RP组和RF组,每组10只。除空白组,其余各组小鼠均自由饮用3%DSS溶液,从造模第4天开始灌胃,连续干预10 d。计算疾病活动指数(disease activity index,DAI),测量小鼠结肠长度,血清中内毒素(lipopolysaccharide,LPS)和D-乳酸(D-lactic acid,D-lac)水平,结肠组织中肿瘤坏死因子-α(tumour necrosis factor-α,TNF-α)、白细胞介素-1β(interleukin-1β,IL-1β)、白细胞介素-6(interleukin-6,IL-6)、白细胞介素-10(interleukin-10,IL-10)、髓过氧化物酶(myeloperoxidase,MPO)、超氧化物歧化酶(superoxide dismutase,SOD)活力和丙二醛(malondialdehyde,MDA)含量;检测结肠组织病理变化及肠道屏障情况;采用实时荧光定量聚合酶链式反应和蛋白质免疫印迹检测结肠NF-κB信号通路中相关基因mRNA和蛋白相对表达量。与模型组相比,RP组和RF组可显著降低结肠炎小鼠DAI,保护结肠组织病理损伤,改善结肠炎小鼠氧化应激损伤,减少炎症因子分泌。RP和RF可减少结肠炎小鼠炎症浸润现象和肠道屏障受损,显著抑制NF-κB信号通路的激活。RP和RF对DSS诱导的UC小鼠具有一定缓解作用,其中RP的效果优于RF。
To investigate the intervention effect and mechanism of Rosa roxburghii Tratt total polysaccharide(RP)and R.roxburghii Tratt total flavonoids(RF)in ulcerative colitis(UC)mice with induced by 3%dextran sodium sulfate(DSS).Forty-eight male mice(C57BL/6J)were randomly divided into control group,model group,RP group and RF group,with 12 mice in each group.Except control group,mice in other groups were given 3%DSS solution freely,and were given intragastric administration from the 4th day of modeling for continuous intervention for 10 days.Calculated the disease activity index(DAI).The length of the colon in mice,serum lipopolysaccharide(LPS),D-lactic acid(D-lac)levels,tumour necrosis factor-α(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6),interleukin-10(IL-10),myeloperoxidase(MPO),superoxide dismutase(SOD)activity and malondialdehyde(MDA)content in the colon tissue of UC mice were determined.Pathological changes in colonic tissue and the intestinal barrier were detected.Quantitative real-time polymerase chain reaction and Western blotting were used to detect the relative expression of relevant genes mRNA and protein in the colon NF-κB signaling pathway.Compared with the model group,the RP and RF groups significantly reduced the DAI of colitis mice,protected the pathological damage of colon tissue,improved oxidative stress damage in colitis mice,and reduced the secretion of inflammatory factors.RP and RF reduced inflammatory infiltration and impaired intestinal barrier in mice with colitis,significantly inhibiting the activation of the NF-κB signaling pathway.RP and RF had a relieving effect on DSS-induced UC mice,where the effect of RP was superior to that of RF.
作者
袁梦
谭书明
任廷远
徐青
谭芸芸
陈珍
陶芸
浦贤
YUAN Meng;TAN Shuming;REN Tingyuan;XU Qing;TAN Yunyun;CHEN Zhen;TAO Yun;PU Xian(College of Brewery and Food Engineering,Guizhou University,Guiyang 550025,China;Guizhou Roxburgh Rose Research Institute,Guiyang 550025,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2022年第24期172-180,共9页
Food and Fermentation Industries
基金
贵州省农业重大产业科学研究攻关项目(黔教合KY字[2019]008)
贵州省林业局科技项目(黔林科合[2020]11)。
关键词
刺梨多糖
刺梨黄酮
溃疡性结肠炎
炎症因子
NF-ΚB信号通路
Rosa roxburghii Tratt polysaccharide(RP)
Rosa roxburghii Tratt flavonoid(RF)
ulcerative colitis
inflammatory factors
NF-κB signaling pathway
