摘要
目的观察并探讨全蝎软膏对兔耳早期增生性瘢痕(hypertrophic scar,HS)的治疗效果。方法自2019年10月至2020年10月,北部战区总医院烧伤整形科将8只健康新西兰大白兔随机分为对照组和治疗组,每组4只,建立兔耳HS模型,建模成功后治疗组即刻采用全蝎软膏进行治疗,模型组采用PBS处理。于治疗后的第7天和第28天取各组动物皮肤织进行HE、MASSON和免疫组化染色检测。结果在造模后28 d,各组兔耳创面均愈合完全并形成色红、触及质硬、突起的HS,建模组瘢痕仍维持明显的增生状态;治疗组瘢痕体积慢慢变小,质地、色泽均有好转。HE和MASSON染色检测结果显示,经全蝎软膏治疗后组织纤维化程度明显减轻(P<0.05);免疫组化检测结果表明,与对照组相比,治疗组第7天和第28天的CD31和血管内皮生长因子(vascular endothelial growth factor,VEGF)的表达量均显著降低(P<0.05)。结论全蝎软膏可以通过降低纤维化程度,下调CD31和VEGF的表达,进而发挥治疗HS的作用。
Objective To observe and investigate the therapeutic effect of scorpion ointment on early hypertrophic scar in rabbit ear model.Methods From October 2019 to October 2020,8 New Zealand white rabbits were randomly divided into control group and treatment group,4 rabbits in each group.The rabbit ear hypertrophic scar model was established.The scorpion ointment was used in the treatment group and the PBS was used in the control group.At 7 and 28 days after treatment,the tissues of each group were taken for HE staining,Masson’s trichrome staining and immunohistochemistry.Results At 28 days after rabbit ear model established,the wounds of rabbit ears in each group were completely healed and hypertrophic scar was formed.Significant hyperplasia of scars was found in the control group.In the treatment group,the scar volume was gradually decreased,and the texture and color were improved.The results of HE staining and Masson’s trichrome staining showed that the degree of tissue fibrosis was significantly reduced after treatment with scorpion ointment(P<0.05).Compared with the control group,the immunohistochemistry results showed that the expression of CD31 and vascular endothelial growth factor(VEGF)in the treatment group at 7 and 28 days were significantly decreased(P<0.05).Conclusion Scorpion ointment can treat hypertrophic scar by reducing the degree of fibrosis and down regulating the expression of CD31 and VEGF.
作者
柴筠
白泽明
刘芮嫡
陶凯
CHAI Jun;BAI Zeming;LIU Ruidi;TAO Kai(Department of Plastic Surgery,Suzhou Hospital Affiliated to Nanjing Medical University,Suzhou 215002,China)
出处
《中国美容整形外科杂志》
CAS
2022年第11期672-676,共5页
Chinese Journal of Aesthetic and Plastic Surgery
基金
国家自然科学基金面上项目(81971845)
辽宁省科学技术计划项目(2021JH2/10300108)。
关键词
瘢痕
全蝎软膏
CD31
血管内皮生长因子
Hypertrophic scar
Scorpion ointment
CD31
Vascular endothelial growth factor
