摘要
单细胞测序技术正逐渐成为生物学基础研究的“必备工具”,为我们理解各种生物学现象带来革命性的洞见。很多传染性疾病均涉及免疫细胞的差异化功能,而这些免疫细胞之间具有较大的异质性。与传统的批量高通量测序相比,近年来新兴的单细胞转录组测序使得研究者能够分析感染过程的免疫细胞异质性,充分挖掘珍贵的临床样本的分子信息,还能获取难以培养的病原微生物的遗传信息。本文着重介绍了当前单细胞测序在传染性疾病及病原微生物研究领域中的应用情况,并对其发展前景做了简要展望。
Single-cell sequencing is becoming an essential tool for the basic research of biology,which brings revolutionary insights for us to understand biological phenomena.Many infectious diseases involve diversified functions of immune cells with great heterogeneity.Compared with high-throughput sequencing,the emerging single-cell transcriptome sequencing enables researchers to explore immune cell heterogeneity during infection,to fully excavate molecular information from precious clinical samples,and to obtain the genetic information of unculturable pathogenic microorganisms.Herein,we review the application status of single-cell sequencing in the research on infectious diseases and pathogenic microorganisms and make a brief prospect of this technology.
作者
陈碧清
刘细细
朱学军
CHEN Biqing;LIU Xixi;ZHU Xuejun(Central Laboratory,Jiangsu Province Hospital of Chinese Medicine/Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing 210029,Jiangsu,China;The First Clinical Medical College,Nanjing University of Chinese Medicine,Nanjing 210029,Jiangsu,China;Department of Hematology,Jiangsu Province Hospital of Chinese Medicine/Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing 210029,Jiangsu,China)
出处
《微生物学通报》
CAS
CSCD
北大核心
2022年第11期4886-4892,共7页
Microbiology China
基金
国家自然科学基金(82001206,81673771)
江苏省中医院高峰学术人才培养工程项目(y2021rc42)
医学免疫学国家重点实验室开放课题(NKMI2021K18)
江苏省中医药管理局科技项目(ZT202106)。
关键词
单细胞测序
传染病
微生物
免疫图谱
宿主-微生物互作
single-cell sequencing
infectious diseases
microorganisms
immune landscape
host-microbe interaction
