摘要
基于HPLC检测分析法,以油菜蜂花粉为研究对象,分析蜂花粉提取物成分,采用Swiss Target Prediction数据库预测成分潜在作用靶点,Gene Cards、OMIM数据库获取抗氧化相关靶点,STRING数据库构建靶点蛋白相互作用PPI网络,Cytoscape 3.8.2软件进行“成分-靶点”的构建与分析,获取网络中的关键靶点与其对应成分,DAVID数据库进行GO分析和KEGG信号通路富集分析.结果表明,对提取物中各成分能实现较好的分离,鉴定出三种黄酮类物质分别为芦丁、绿原酸和异鼠李素,其中芦丁在2.81-281μg/mL(r^(2)=0.9952),绿原酸在1.63-163μg/mL(r^(2)=0.9998),异鼠李素在1.13-113.2μg/mL(r^(2)=0.9999)时线性关系良好.网络药理学分析表明,蜂花粉黄酮类成分芦丁、绿原酸、异鼠李素等成分通过作用于PTGS2、EGFR、SRC、TNF、AKT1、PARP1、MMP9、AKR1B1、CA9等靶点.通过氮代谢、EGFR酪氨酸激酶抑制剂抗性、VEGF信号通路、非小细胞肺癌、ErbB信号通路、糖尿病并发症中的AGE-RAGE信号通路、C-型凝集素受体信号通路、FoxO信号通路、磷脂酶D信号通路、粘着点、PI3K-Akt信号通路等信号通路发挥抗氧化作用,为蜂花粉综合利用提供理论基础.
In this study,we analyzed the components of bee pollen extracts based on HPLC assay,and used Swiss Target Prediction database to predict the potential targets of components,Gene Cards and OMIM database to obtain antioxidant-related targets,and STRING database to construct PPI network of target protein interactions.Cytoscape 3.8.2 software was used to construct and analyze the“component-target”network to obtain the key targets in the network and their corresponding components,and DAVID database was used for GO analysis and KEGG signaling pathway enrichment analysis.The results showed that the components of the extract were well separated,and three flavonoids were identified as Rutin,Chlorogenic Acid and Isorhamnetin,with Rutin in the range of 2.81-281μg/mL(r^(2)=0.9952),chlorogenic acid in the range of 1.63-163μg/mL(r^(2)=0.9998)and isorhamnetin in the range of 1.13-113.2μg/mL(r^(2)=0.9999).With good linearity in the range.Network pharmacological analysis showed that the bee pollen flavonoid components Rutin,Chlorogenic acid,and Isorhamnetin acted through the targets of PTGS2,EGFR,SRC,TNF,AKT1,PARP1,MMP9,AKR1B1,and CA9.Antioxidant effects through nitrogen metabolism,EGFR tyrosine kinase inhibitor resistance,VEGF signaling pathway,non-small cell lung cancer,ErbB signaling pathway,AGE-RAGE signaling pathway in diabetic complications,C-type lectin receptor signaling pathway,FoxO signaling pathway,phospholipase D signaling pathway,adhesion sites,PI3K-Akt signaling pathway,etc.,Theoretical basis for the comprehensive utilization of bee pollen.
作者
刘慧琴
陈远
刘心雨
郑梦晴
段红
翟科峰
LIU Hui-qin;CHEN Yuan;LIU Xin-yu;ZHEN Meng-qing;DUAN Hong;ZHAI Ke-feng(Suzhou University,Suzhou,Anhui,234000,China;Engineering Research Center for Development and High Value Utilization of Genuine Medicinal Materials in North Anhui Province,Suzhou University,Suzhou,Anhui,234000,China;Anhui Polytechnic Uni-versity,Wuhu,Anhui,241000,China)
出处
《凯里学院学报》
2022年第6期28-36,共9页
Journal of Kaili University
基金
安徽省自然科学基金(2008085J39,2108085MH314)
高校学科(专业)拔尖人才学术资助项目(gxbjZD2022073)
药学重点学科(2019xjzdxk2)
国家级大学生创新训练计划项目(SZ2017ZX06)。
