摘要
建立了高效液相色谱(HPLC)测定追风活血膏中士的宁、乌头碱、马钱子碱、当归、乳香、没药、蜕皮甾酮和青藤碱8种成分含量的方法。色谱柱为Agilent ZORBAXSB-C_(18)柱(4.6 mm×250 mm,5μm);流动相:甲醇(A相),0.1%H_(3)PO_(4)水溶液(B相);流速1.0 mL/min;柱温室温;检测波长256 nm。结果显示,8种成分能够达到良好分离,在各自线性范围内线性关系良好,相关系数r均大于0.999;平均加样回收率在98.74%~99.67%之间,相对标准偏差(RSD)在0.85%~1.76%范围内。5批次样品中士的宁、乌头碱、马钱子碱、当归、乳香、没药、蜕皮甾酮和青藤碱的含量分别为0.031~0.349,0.021~0.166,0.024~0.298,0.021~0.219,0.017~0.186,0.015~0.158,0.015~0.149,0.016~0.179 mg/g。本文所建立的HPLC法操作简单、稳定可控,可用于追风活血膏的质量控制。
A high performance liquid chromatographic(HPLC)method was developed for determination of the eight components in Zhuifeng Huoxue Ointment,namely,brucine,aconitine,strychnine,angelica,frankincense,myrrh,ecdysterone and sinomenine.The chromatographic column was an Agilent ZORBAXSB-C_(18) column(4.6 mm×250 mm,5μm);the mobile phase was methanol(phase A)and 0.1%aqueous solution of H_(3)PO_(4)(phase B);the flow rate was 1.0 m L/min;the column temperature was room temperature;the detection wavelength was 256 nm.The average recoveries ranged from 98.74%-99.67%,and the relative standard deviations(RSDs)were in the range of 0.85%-1.76%.The content of strychnine,aconitine,brucine,angelica,frankincense,myrrh,ecdysterone and sinomenine in the five batches of samples were 0.031-0.349,0.021-0.166,0.024-0.298,0.021-0.219,0.017-0.186,0.015-0.158,0.015-0.149,0.016-0.179 mg/g,respectively.The HPLC method developed is simple,stable and controllable,and can be used for the quality control of Zhuifeng Huoxue Ointment.
作者
万刘静
张利
Liujing Wan;Li Zhang(School of Health,Chongqing Industry and Trade Vocational and Technical College,Fuling,Chongqing 408000,China;School of Chemical Engineering,Sichuan University of Science&Engineering,Zigong,Sichuan 643002,China)
出处
《日用化学工业（中英文）》
CAS
北大核心
2022年第12期1342-1347,共6页
China Surfactant Detergent & Cosmetics
基金
四川省科技厅支撑计划项目(2019YFS0095)。
关键词
追风活血膏
高效液相色谱法
质量控制
Zhuifeng Huoxue Ointment
high performance liquid chromatography
quality control
