摘要
随着分子生物学的迅猛发展,农杆菌介导技术在转化机制、影响因素、转化率等方面的优势日益为世人所知,其应用也由原来的双子叶植物逐步过渡到以禾本科作物为主的单子叶植物。基于农杆菌转化的机制,回顾了农杆菌转化技术在小麦、玉米和水稻这3种主要作物上的应用现状,总结了影响转化率的因素并提出了提高转化率的对策,以期为农杆菌转化单子叶植物技术的进一步改进与优化提供有效的借鉴。
With the rapid develpoment of molecular biology,Agrobacterium-mediated technology has become increasingly known to the world in the transformation mechanism,influencing factors,conversion rate and other aspects,and its application has gradually transferred from the original dicotyledons to monocotyledons dominated by gramineous crops.Based on the mechanism of Agrobacterium transformation,this paper reviewed the application status of Agrobacterium transformation technology in wheat,maize,and rice,briefly summarized the factors affecting the conversion rate and put forward the countermeasures to improve the conversion rate,in order to provide effective reference for the further improvement and optimization of Agrobacterium transformation of monocotyledons.
作者
刘文欣
LIU Wenxin(College of Life Sciences,Shandong Agricultural University,Taian 271018,China)
出处
《种业导刊》
2022年第6期11-16,共6页
Journal of Seed Industry Guide
关键词
农杆菌介导
单子叶植物
遗传转化
小麦
玉米
水稻
Agrobacterium mediation
Monocotyledons
Genetic transformation
Wheat
Corn
Rice
