摘要
目的:探究胆汁淤积性肝病婴儿肠道微生态与胆汁酸代谢的关系,为临床诊治及预防提供依据。方法:选取51例婴儿胆汁淤积性肝病患儿为观察组,并随机选取同期57例健康婴儿作为健康对照组。采集两组婴儿粪便标本,通过荧光定量PCR法测定其肠道菌群多样性,定量检测DNA-A260、双歧杆菌、乳酸杆菌、大肠杆菌数量,计算双歧杆菌/大肠杆菌数值比值(B/E值)。比较两组研究对象肠道微生态指标及血清总胆汁酸(TBA)水平,通过Pearson积矩相关分析评估婴儿胆汁淤积性肝病患儿的B/E值与TBA指标间相关性。观察组患儿根据是否发生肝硬化分为肝硬化组和无肝硬化组,比较组间肠道微生态指标,采用Spearman相关分析婴儿胆汁淤积性肝病患儿病情严重程度与肠道微生态指标相关性。结果:观察组患儿TBil、DBil、ALT、AST、ALP、GGT、血氨、TC、大肠杆菌数量、TBA水平明显高于健康对照组(P<0.05);DNA-A260、双歧杆菌、乳酸杆菌数量及B/E值明显低于健康对照组(P<0.05);经Pearson相关分析,婴儿胆汁淤积性肝病患儿B/E值与TBA水平具有负相关(r=-0.838,P<0.05);肝硬化组患者双歧杆菌数量及B/E明显低于无肝硬化组(P<0.05);经Spearman相关分析,婴儿胆汁淤积性肝病患儿病情严重程度与双歧杆菌数量及B/E值具有负相关性(P<0.05)。结论:婴儿胆汁淤积性肝病患儿体内确存在肠道菌群失衡的情况,并伴随着胆汁酸代谢异常,且两者具有一定相关性。
Objective:To explore the relationship between intestinal microecology and bile acid metabolism in infants with cholestatic liver disease,so as to provide basis for clinical diagnosis,treatment and prevention.Methods:51 infants with infantile cholestatic liver disease were selected as observation group,and 57 healthy infants during the same period were randomly selected as healthy control group.Two sets of infant stool samples were collected and the diversity of intestinal flora was detected by fluorescence quantitative PCR,while the counts of DNA-A260,Bifidobacterium,Lactobacillus and Escherichia coli were quantitatively detected,and the ratio of Bifidobacterium to Escherichia coli(B/E value)was calculated.The intestinal microecological indicators and serum total bile acid(TBA)level were compared between the two groups,and the correlation between B/E value and TBA in infants with infantile cholestatic liver disease was assessed by Pearson product-moment correlation analysis.The observation group was divided into cirrhosis group and non-cirrhosis group according to the presence or absence of cirrhosis.The intestinal microecological indexes were compared between the two groups.Spearman correlation was used to analyze the correlation between the severity of infantile cholestatic liver disease and intestinal microecological indexes.Results:The TBil,DBil,ALT,AST,ALP,GGT,blood ammonia,TC,count of Escherichia coli and TBA in observation group were significantly higher than those in healthy control group(P<0.05).The counts of DNA-A260,Bifidobacterium and Lactobacillus and B/E value were significantly lower than those of healthy control group(P<0.05).According to Pearson correlation analysis,the B/E value of infants with infantile cholestatic liver disease was negatively correlated with TBA level(r=-0.838,P<0.05).The count of Bifidobacterium and B/E value in cirrhosis group were significantly lower than those in non-cirrhosis group(P<0.05).Spearman correlation analysis showed that the disease severity in infants with infa
作者
高玲
封东进
王光猛
董中茂
周琦
孙蓓蓓
GAO Ling;FENG Dong-jin;WANG Guang-meng(Department of gastroenterology,Xuzhou Children's Hospital,Xuzhou Medical University,Xuzhou Jiangsu,221000 China)
出处
《中西医结合肝病杂志》
CAS
2022年第12期1093-1096,共4页
Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases
关键词
肠道微生态
胆汁酸代谢
婴儿胆汁淤积性肝病
相关性
intestinal microecology
bile acid metabolism
infantile cholestatic liver disease
correlation
